	   SnapGene      9êTACCATGGTCAAGAGTCCCCCGTGTTCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTTTTGGAGTAGACAAGTGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCCATGAAGCTAAACTGAAGGCGGGAAACGACAATCTGATCCAAGCTCAAGCTGCTCTAGCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCCAAGCTTGCATGCCTGCAGTGCAGCGTGACCCGGTCGTGCCCCTCTCTAGAGATAATGAGCATTGCATGTCTAAGTTATAAAAAATTACCACATATTTTTTTTGTCACACTTGTTTGAAGTGCAGTTTATCTATCTTTATACATATATTTAAACTTTACTCTACGAATAATATAATCTATAGTACTACAATAATATCAGTGTTTTAGAGAATCATATAAATGAACAGTTAGACATGGTCTAAAGGACAATTGAGTATTTTGACAACAGGACTCTACAGTTTTATCTTTTTAGTGTGCATGTGTTCTCCTTTTTTTTTGCAAATAGCTTCACCTATATAATACTTCATCCATTTTATTAGTACATCCATTTAGGGTTTAGGGTTAATGGTTTTTATAGACTAATTTTTTTAGTACATCTATTTTATTCTATTTTAGCCTCTAAATTAAGAAAACTAAAACTCTATTTTAGTTTTTTTATTTAATAATTTAGATATAAAATAGAATAAAATAAAGTGACTAAAAATTAAACAAATACCCTTTAAGAAATTAAAAAAACTAAGGAAACATTTTTCTTGTTTCGAGTAGATAATGCCAGCCTGTTAAACGCCGTCGACGAGTCTAACGGACACCAACCAGCGAACCAGCAGCGTCGCGTCGGGCCAAGCGAAGCAGACGGCACGGCATCTCTGTCGCTGCCTCTGGACCCCTCTCGAGAGTTCCGCTCCACCGTTGGACTTGCTCCGCTGTCGGCATCCAGAAATTGCGTGGCGGAGCGGCAGACGTGAGCCGGCACGGCAGGCGGCCTCCTCCTCCTCTCACGGCACGGCAGCTACGGGGGATTCCTTTCCCACCGCTCCTTCGCTTTCCCTTCCTCGCCCGCCGTAATAAATAGACACCCCCTCCACACCCTCTTTCCCCAACCTCGTGTTGTTCGGAGCGCACACACACACAACCAGATCTCCCCCAAATCCACCCGTCGGCACCTCCGCTTCAAGGTACGCCGCTCGTCCTCCCCCCCCCCCCCTCTCTACCTTCTCTAGATCGGCGTTCCGGTCCATGGTTAGGGCCCGGTAGTTCTACTTCTGTTCATGTTTGTGTTAGATCCGTGTTTGTGTTAGATCCGTGCTGCTAGCGTTCGTACACGGATGCGACCTGTACGTCAGACACGTTCTGATTGCTAACTTGCCAGTGTTTCTCTTTGGGGAATCCTGGGATGGCTCTAGCCGTTCCGCAGACGGGATCGATTTCATGATTTTTTTTGTTTCGTTGCATAGGGTTTGGTTTGCCCTTTTCCTTTATTTCAATATATGCCGTGCACTTGTTTGTCGGGTCATCTTTTCATGCTTTTTTTTGTCTTGGTTGTGATGATGTGGTCTGGTTGGGCGGTCGTTCTAGATCGGAGTAGAATTCTGTTTCAAACTACCTGGTGGATTTATTAATTTTGGATCTGTATGTGTGTGCCATACATATTCATAGTTACGAATTGAAGATGATGGATGGAAATATCGATCTAGGATAGGTATACATGTTGATGCGGGTTTTACTGATGCATATACAGAGATGCTTTTTGTTCGCTTGGTTGTGATGATGTGGTGTGGTTGGGCGGTCGTTCATTCGTTCTAGATCGGAGTAGAATACTGTTTCAAACTACCTGGTGTATTTATTAATTTTGGAACTGTATGTGTGTGTCATACATCTTCATAGTTACGAGTTTAAGATGGATGGAAATATCGATCTAGGATAGGTATACATGTTGATGTGGGTTTTACTGATGCATATACATGATGGCATATGCAGCATCTATTCATATGCTCTAACCTTGAGTACCTATCTATTATAATAAACAAGTATGTTTTATAATTATTTTGATCTTGATATACTTGGATGATGGCATATGCAGCAGCTATATGTGGATTTTTTTAGCCCTGCCTTCATACGCTATTTATTTGCTTGGTACTGTTTCTTTTGTCGATGCTCACCCTGTTGTTTGGTGTTACTTCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCCGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCCTAAACTGCATTTCACCAGCCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGGAGACTAGTGAGCTCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGCTAGAGCAGCTTGCCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTGAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCGAGCTTTCGCAGATCCCGGGGGGCAATGAGATATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAGTTTAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTACAACCGGTCGCGGAGGCTATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCACGACTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGAAATAGAGTAGATGCCGACCGGATCTGTCGATCGACAAGCTCGAGTTTCTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTACTAAAATCCAGATCCCCCGAATTAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTGATCACCGCGGTTTCAAAATCGGCTCCGTCGATACTATGTTATACGCCAACTTTGAAAACAACTTTGAAAAAGCTGTTTTCTGGTATTTAAGGTTTTAGAATGCAAGGAACAGTGAATTGGAGTTCGTCTTGTTATAATTAGCTTCTTGGGGTATCTTTAAATACTGTAGAAAAGAGGAAGGAAATAATAAATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCGCTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAAGGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAGTATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTGGCCGATGTGGATTGCGAAAACTGGGAAGAAGACACTCCATTTAAAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTGTCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGATCAGGGAGGATATCGGGGAAGAACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGTACCTAGAATGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGGGTGCCTTGATGTGGGCGCCGGCGGTCGAGTGGCGACGGCGCGGCTTGTCCGCGCCCTGGTAGATTGCCTGGCCGTAGGCCAGCCATTTTTGAGCGGCCAGCGGCCGCGATAGGCCGACGCGAAGCGGCGGGGCGTAGGGAGCGCAGCGACCGAAGGGTAGGCGCTTTTTGCAGCTCTTCGGCTGTGCGCTGGCCAGACAGTTATGCACAGGCCAGGCGGGTTTTAAGAGTTTTAATAAGTTTTAAAGAGTTTTAGGCGGAAAAATCGCCTTTTTTCTCTTTTATATCAGTCACTTACATGTGTGACCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGGGTTCCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGTGCTATCCACAGGAAAGAGACCTTTTCGACCTTTTTCCCCTGCTAGGGCAATTTGCCCTAGCATCTGCTCCGTACATTAGGAACCGGCGGATGCTTCGCCCTCGATCAGGTTGCGGTAGCGCATGACTAGGATCGGGCCAGCCTGCCCCGCCTCCTCCTTCAAATCGTACTCCGGCAGGTCATTTGACCCGATCAGCTTGCGCACGGTGAAACAGAACTTCTTGAACTCTCCGGCGCTGCCACTGCGTTCGTAGATCGTCTTGAACAACCATCTGGCTTCTGCCTTGCCTGCGGCGCGGCGTGCCAGGCGGTAGAGAAAACGGCCGATGCCGGGATCGATCAAAAAGTAATCGGGGTGAACCGTCAGCACGTCCGGGTTCTTGCCTTCTGTGATCTCGCGGTACATCCAATCAGCTAGCTCGATCTCGATGTACTCCGGCCGCCCGGTTTCGCTCTTTACGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGATACCGTCACCAGGCGGCCGTTCTTGGCCTTCTTCGTACGCTGCATGGCAACGTGCGTGGTGTTTAACCGAATGCAGGTTTCTACCAGGTCGTCTTTCTGCTTTCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGTCCGCAACGTGTGGACGGAACACGCGGCCGGGCTTGTCTCCCTTCCCTTCCCGGTATCGGTTCATGGATTCGGTTAGATGGGAAACCGCCATCAGTACCAGGTCGTAATCCCACACACTGGCCATGCCGGCCGGCCCTGCGGAAACCTCTACGTGCCCGTCTGGAAGCTCGTAGCGGATCACCTCGCCAGCTCGTCGGTCACGCTTCGACAGACGGAAAACGGCCACGTCCATGATGCTGCGACTATCGCGGGTGCCCACGTCATAGAGCATCGGAACGAAAAAATCTGGTTGCTCGTCGCCCTTGGGCGGCTTCCTAATCGACGGCGCACCGGCTGCCGGCGGTTGCCGGGATTCTTTGCGGATTCGATCAGCGGCCGCTTGCCACGATTCACCGGGGCGTGCTTCTGCCTCGATGCGTTGCCGCTGGGCGGCCTGCGCGGCCTTCAACTTCTCCACCAGGTCATCACCCAGCGCCGCGCCGATTTGTACCGGGCCGGATGGTTTGCGACCGTCACGCCGATTCCTCGGGCTTGGGGGTTCCAGTGCCATTGCAGGGCCGGCAGACAACCCAGCCGCTTACGCCTGGCCAACCGCCCGTTCCTCCACACATGGGGCATTCCACGGCGTCGGTGCCTGGTTGTTCTTGATTTTCCATGCCGCCTCCTTTAGCCGCTAAAATTCATCTACTCATTTATTCATTTGCTCATTTACTCTGGTAGCTGCGCGATGTATTCAGATAGCAGCTCGGTAATGGTCTTGCCTTGGCGTACCGCGTACATCTTCAGCTTGGTGTGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCATGGCTGGCGTGTCTGCCAGGCTGGCCAACGTTGCAGCCTTGCTGCTGCGTGCGCTCGGACGGCCGGCACTTAGCGTGTTTGTGCTTTTGCTCATTTTCTCTTTACCTCATTAACTCAAATGAGTTTTGATTTAATTTCAGCGGCCAGCGCCTGGACCTCGCGGGCAGCGTCGCCCTCGGGTTCTGATTCAAGAACGGTTGTGCCGGCGGCGGCAGTGCCTGGGTAGCTCACGCGCTGCGTGATACGGGACTCAAGAATGGGCAGCTCGTACCCGGCCAGCGCCTCGGCAACCTCACCGCCGATGCGCGTGCCTTTGATCGCCCGCGACACGACAAAGGCCGCTTGTAGCCTTCCATCCGTGACCTCAATGCGCTGCTTAACCAGCTCCACCAGGTCGGCGGTGGCCCATATGTCGTAAGGGCTTGGCTGCACCGGAATCAGCACGAAGTCGGCTGCCTTGATCGCGGACACAGCCAAGTCCGCCGCCTGGGGCGCTCCGTCGATCACTACGAAGTCGCGCCGGCCGATGGCCTTCACGTCGCGGTCAATCGTCGGGCGGTCGATGCCGACAACGGTTAGCGGTTGATCTTCCCGCACGGCCGCCCAATCGCGGGCACTGCCCTGGGGATCGGAATCGACTAACAGAACATCGGCCCCGGCGAGTTGCAGGGCGCGGGCTAGATGGGTTGCGATGGTCGTCTTGCCTGACCCGCCTTTCTGGTTAAGTACAGCGATAACCTTCATGCGTTCCCCTTGCGTATTTGTTTATTTACTCATCGCATCATATACGCAGCGACCGCATGACGCAAGCTGTTTTACTCAAATACACATCACCTTTTTAGACGGCGGCGCTCGGTTTCTTCAGCGGCCAAGCTGGCCGGCCAGGCCGCCAGCTTGGCATCAGACAAACCGGCCAGGATTTCATGCAGCCGCACGGTTGAGACGTGCGCGGGCGGCTCGAACACGTACCCGGCCGCGATCATCTCCGCCTCGATCTCTTCGGTAATGAAAAACGGTTCGTCCTGGCCGTCCTGGTGCGGTTTCATGCTTGTTCCTCTTGGCGTTCATTCTCGGCGGCCGCCAGGGCGTCGGCCTCGGTCAATGCGTCCTCACGGAAGGCACCGCGCCGCCTGGCCTCGGTGGGCGTCACTTCCTCGCTGCGCTCAAGTGCGCGGTACAGGGTCGAGCGATGCACGCCAAGCAGTGCAGCCGCCTCTTTCACGGTGCGGCCTTCCTGGTCGATCAGCTCGCGGGCGTGCGCGATCTGTGCCGGGGTGAGGGTAGGGCGGGGGCCAAACTTCACGCCTCGGGCCTTGGCGGCCTCGCGCCCGCTCCGGGTGCGGTCGATGATTAGGGAACGCTCGAACTCGGCAATGCCGGCGAACACGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTACGCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTCCAGTAGGTCGCGGGTGCTGCGGGCCAGGCGGTCTAGCCTGGTCACTGTCACAACGTCGCCAGGGCGTAGGTGGTCAAGCATCCTGGCCAGCTCCGGGCGGTCGCGCCTGGTGCCGGTGATCTTCTCGGAAAACAGCTTGGTGCAGCCGGCCGCGTGCAGTTCGGCCCGTTGGTTGGTCAAGTCCTGGTCGTCGGTGCTGACGCGGGCATAGCCCAGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTATTCTACTTTATGCGACTAAAACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCAGCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGAACGTCAGAAGCCGACTGCACTATAGCAGCGGAGGGGTTGGATCAAAGTACTTTGATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGTTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACACTGATAGTTTAATTCCCGATCTAGTAACATAGATGACACCGCGCGCGATAATTTATCCTAGTTTGCGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAAAAACCCATCTCATAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAATTCGAGCTGGTCACCTGTAATTCACACGTGGTGGTGGTGGTGGTGGCTAGCTTGTTTGCCTCCCTGCTGCGGTTTTTCACCGAAGTTCATGCCAGTCCAGCGTTTTTGCAGCAGAAAAGCCGCCGACTTCGGTTTGCGGTCGCGAGTGAAGATCCCTTTCTTGTTACCGCCAACGCGCAATATGCCTTGCGAGGTCGCAAAATCGGCGAAATTCCATACCTGTTCACCGACGACGGCGCTGACGCGATCAAAGACGCGGTGATACATATCCAGCCATGCACACTGATACTCTTCACTCCACATGTCGGTGTACATTGAGTGCAGCCCGGCTAACGTATCCACGCCGTATTCGGTGATGATAATCGGCTGATGCAGTTTCTCCTGCCAGGCCAGAAGTTCTTTTTCCAGTACCTTCTCTGCCGTTTCCAAATCGCCGCTTTGGACATACCATCCGTAATAACGGTTCAGGCACAGCACATCAAAGAGATCGCTGATGGTATCGGTGTGAGCGTCGCAGAACATTACATTGACGCAGGTGATCGGACGCGTCGGGTCGAGTTTACGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGACAGCTGAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGTAAGTCCGCATCTTCATGACGACCAAAGCCAGTAAAGTAGAACGGTTTGTGGTTAATCAGGAACTGTTGGCCCTTCACTGCCACTGACCGGATGCCGACGCGAAGCGGGTAGATATCAGACTCTGTCTGGCTTTTGGCTGTGACTTCGAGTTCATAGAGATAACCTTCACCCGGTTGCCAGAGGTGCGGATTCACCACTTGCAAAGTCCCGCTAGTGCCTTGTCCAGTTGCAACCACCTGTTGATCCGCATCACGCAGTTCAACGCTGACATCACCATTGGCCACCACCTGCCAGTCAACAGACGCGTGGTTACAGTCTTGCGCGACATGCGTCACCACGGTGATATCGTCCACCCAGGTGTTCGGCGTGGTGTAGAGCATTACGCTGCGATGGATTCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTTCGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACACAAACGGTGATACGTACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATTACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGATCAATTCCACAGTTTTCGCGATCCAGACTGAATGCCCACAGGCCGTCGAGTTTTTTGATTTCACGGGTTGGGGTTTCTACAGGACGGACGAGTCGTCGGTTCTGTAACTATCATCATCATCATAGACACACGAAATAAAGTAATCAGATTATCAGTTAAAGCTATGTAATATTTACACCATAACCAATCAATTAAAAAATAGATCAGTTTAAAGAAAGATCAAAGCTCAAAAAAATAAAAAGAGAAAAGGGTCCTAACCAAGAAAATGAAGGAGAAAAACTAGAAATTTACCCTCAGATC  yÕ   +   0   <   9   0   C   =   %   9   ,   N   :   X   A   9   U   !   &   (   7   6   O   %   ,   V   :   1   O   8   7   $   +   :   ,   -   =   %   0   -   (   9   )   S   H   K   6   )   '   g   <   E   L   G   0   =   1   M   )   K   A   b   T   O   7   2   /       )   %   :   7   6   +   '   6      >   0   E   6   3   D   ,   ,   N   h   F   <   3   B   6   8   3   -   /   )   7   4   9   I   (   B   H   7   -   -   J   0   G   N   A   8   @   ,   *   <   3   *   <   1   5      1   )   :   7   1   !   *   6   D   E   (   -   Q   0   8   /   I   3   M   ?   <   a      /      %   #   @   D   !   '   9   :   ?   +   4   $   (   :   ?   :   P   %   4   5   -   Q   N   “   \   9   X   K   M   X   8   6   ?   5   >   H   C   =   2   U   6   8   >   <   5   I   @   M   €   )   ,   K   3   4   (   0   E   Q   5   H   P   7   3   8   a   (   A   4   /   =   4   3   I   <   2   0   G   >   7   C   S      "   /      L      ^   U   :   "   E   ;   W   3   ,   W   T   4   ,   @   L      E   <   9   +   )   ,        + ¹ º «¬=>¬¾¿ª1žéêëè’³–¼�‘’67‡ˆ‰ð!Q$ô&'€'Í'�*+Ÿ-¯-°-0<0Î190 Q Ç 7y­?­¶ÐšÀÍqŸ*qcé“E�Fw�¹Q‹—½Íñ+89!ø!<$¸%Æ&�)+ñ-=0-1Ï1j7< > µ » ÇÒovõ+ûÜ	å
�«[2 g‰�]MX×ó´[“WÔ n!
"I"·"Æ"á"R$g$c&�'Y)„)S+C- -¦-ß-�. 0û384a465ü5ý89µ99 :~‹Œ#`<	Ô[—OìQvVˆì^‡Š!|!ñ!#w#†%š&õ&'U'y'”'Î'I)ª)J*‘*Ñ*+D+J+^,ã,-‹-«-±-Ä-Š.³.Ô.A/·/¼/0 m 	J™ý¡rV	J›¹¶ ÍÎ¥NsêS�·ÍçñyŽð–GÛ9RŒÎ«ò,Ž:!ù!0J5Ë9C : • A8z+®Öƒ	·	”Îr+iŽØr°Õ”Ø™ÐFÀ‰ÏÓõ"&ˆŽ¦ªºðÿ˜9—F L q"ü"@#y%¹%õ%&?&z&Y*+-ò-ô.±/>0Ð1Í557= J R T®@s·Ñ�ŸÒå³	Æ	V
s
Ý
Áq¤7ÿ~Fd|\F”ûä]Š«�¾éG�uõI#r#=$Þ$ª%&Ç&Ò&Z'+<,c.%0e0¢1ë5M67k7% È ïk¸×\¾£ª�é%a€­€gx”b`üu%à(@)ž)y*Õ/]0.1�3ò344999 ¶ †�3	q	Á
(b¬¶3Êm´CŽŠ o!¾#S$%d&ß'(7*±,-n-¡-à-..1.‚.º.â.è. /6/ò1õ1ø1û1þ1Ö2343Ê3ë3b4H6å6÷67�7, ÈÞ)êË	œ
æ
Ž !hŠôqµ7oj»C] w ©!"c#&$€$�%5(U(…)‘,=-§-Ä39475A5F5ý5W768N ‘ MÓpw~öûI\ü%	8	Ý	
§\¨�‚À9NYØ(z‘\”XµL!J"£"¸"Ç"Í"â"]#h$®$,%£%°%‰&¿&^'‚'æ'Z)k)å*ì*T+w+þ+O,ž,¶, /w/!0H0¢02W2¢3@4e4½5Ä5>6Æ6þ899:  ? ¼ z
ç,Wd@	Ð
eÝ²ùMŸv£^¾‚ƒÕ †!¢#7%Í%-&5'[(0*Æ*Õ*]+ð,D-|-å-.{.“.�.½.Ø.$///Ý1^2•2ü3j5¶9X ü4¤»úa=	Õ\¥Rw¯ˆW‰Þ„�ø_F{ù‹}!ƒ!Ž!”!í!ò!"F"n"F#Ù#$$#$ì$›&­&¼&ö&'Ù'f(­(t)w)4*’*Ò*9+E+ô+÷+_,Ë,Ø,ä,-Œ-¬-Ë-
.).�.´.Õ.W/Z/i/½/¡2¿24þ6g7²7Ç7q8A Ó Ù ÿ ;ž£è/4Îö	]
Q‰àp·í¹Ü6ê@íOWo ÈÏ6Z!j!#ž#%X%™'Ï'�(è(J)z)«)Å)+(,-.m.B/¸/À/L0›1¨3H4!7¥7Š89 j»+˜b´	“PÚVMÄµ4à¢×DBÂ9ˆÛ !™!#"±"Û"$È$Ò$P%‡%ž&µ&z'©(º(K*P*j*K+b,>.H.ô/23A3Á466½7š8U è Œò�$Y¹“Æk‡hº¥ñ¢øª¼Çµ!¼!Î!Õ!ß!."A"x#2$¦$ï$ù$‚%à%é%í%F&ß&'!'V'•'³'¾(ä(5)¹)ø):*±*¸*ý*3+
,,,,7,s,¨,Û,ê,-s-²-Å-Î-!.%.7.Q.[.‹.Ê.	//P/X3P5Ö5©6%8å8! 8 × HKÛ
£Ì¨OtT#†¡¸èêñÍïý�)D J!h!¤-0:0“2?5F6& n ‚Û-ÊÃ	/Kœº·V¡W¦óô�éHSÏQÃÚó#*$S,¸3.6®9Ì9(  šÒÿ÷?bÅsW	¨	
j=ëù4—ë
: 8 Š!R"f"b% )<+d23 4˜4K5%68ì897 
5þ¢EÓ�ÀA¹ÎÏØö}‚Îãò3zj‘Ü.Ù¨*¤Ì¬Ó!-l� / @ P ;!Ê!ú!s&H-Ø1Ï3#5	76 ; G ‰ ½&1{p•É•þjßE±&•UÙ/ž¾ÁÐ#§»¾úU™¸îG M è!"&d*¦*-î/0&2h2]3÷567È9O $ e9§»,ð×¸	Šòs,d�Ùs«Ö ³ú(0ÑótÝUaÈÔûJP�«"²ñ/Ñ;Üõ:˜_!r"ý"Á#,$º%ö%@&&–(—,õ.§/²/1°1Ñ1F2¤24Ò4é4-78@9’9°9Â9Î9×9% B Ìð¯xÄ\	Æö#ÏŽè/XšGÔ�‘ëö'l…–íÿ%%¦)Z*+ƒ5d8Ú8, ) – çÙy„	6ÄŠ‰Ë œŒ°¾' A#Ç#Ð#÷#V$\%z%{&g),+g,ó-û-.ý/?0a0Æ1Ù3¹5Î5t8V © å ü ¯t¸ORÌÏÓö		+	.	°	´	×	æ	ó	W
Z
®
X »çKeBmš]æ‹ILb²þ¶åƒšÔõ¦3ê|X ¤ w!J#6$œ$µ%R&'['@(Ô)µ*Ù*P+Z+L-é-d.ß073E3Á35ì57×7: K p 3U�/åDæÇ	M
D€ý¼Û†¥òˆ	9ÏPõ}Ê@i=�ÓIkvÅö Û!ß$J(˜)„+f0Â0î0‰1Ù2ñ2:3606è6l7“78I81 S À¾A�ÖÞ‘t
sòe8 GÒ´ÂG•ÿ'^‹¬âÉsí­!«%È&Ó&ì&K'q'ð'')`)=,ì-£1N67é7õ8O9O F‚·HYÒn ßR	À	G
Þ
1ÂrÐÉã$©boÉüt	Ìè�ÐK†¿@HSžX#s#¯#î#>$B$x$–$½$&Ø&„(ã)s*�*ð*+y,ˆ,€-•-ë.&040Ñ0Ê1k2Ê2º3î3„6y8»8Ð8†9–9å98 Œ ºâï_›Í¯ú‘”Jâ)\hzê^Ö=g×�díhú !‘!ã!v%<&ª&á(Ê+ñ+U,</ñ/^0¹2¼2Å2Ñ2å3ó354£5ò5¿6P8997 /Ÿ>âð lw†ñ
¨ÂÔ]¬¤ó«1âíbh•UvÁcoýæ %!3"8&�'í)ò)z*ß*/,¢/ß/â0Ž3â4ð4�6Ö6·799$ „ÝˆþØÔ
ž¹Y&}�¤´agÐ m#Z&Ÿ)$+•/Ö/0/1Q2¥466=7>8Ö8+ É ¹yM_µ¿Hƒ=ü'‘%®Ky2´+ /!"Ÿ"##ª#e'�(A)*j+Â+—1¦5ç5ð78: O k � ³ · øUŽ1	6	Ú	q
)cËn°Öýµ÷Dz¾ÎD†©@h�7Dóz!Œ!%s%8*-,./.».F0ð1ó1ö1ù1ü1ÿ14c4h5Â5Ä67, -ü	M)	¾ð?N²ä2�í-Ñz > Î -!�"¿#T$v$‚(–)*¢-é.f2×253Í3ì36æ6‘7µ78-  ¤¦Â'˜BMS ©«4	r	�	�	‘	Â
ØŸ·î&Ò¹0e éQD!›"%q%€(°(ò(2.3Ë3ø67= W «‡²÷ s!^­²«­4}_Ü÷><™s ‹ p!'"ð"$%&e&ù&‰'à'Ø(ø(o)~)Û)X,²,7-[-o-�-š-á-ƒ.ã.//7/Û/3)4�4n5I6h6% œA*uY	a¨p»û6õÅ8ëñª!¹!õ!"d#ë#'$Ã$&6(†) *¨-x.·.G5¶5þ570 ×Éèm�õj¡3AÕ("i_‹Ž™<e¼	K$Ñ%V(F*’,l/Í/×0t1å1š3‰42585‘5±597X7ÿ7£8$9H9b9- ˆãù¥ët	‰	Ì	3
!-¯ÔÉ~‚ˆr¶8p3Þk¡Dý^ x ¸ ž%C(�+…/1#1#3.3Å3:4Ê778( ±b–ßm~
�
ç
ëŒÁ¿êø– ­"Â"×"ì"8#—#�$Ÿ$%d%Ç%P'i(p(³)}+m,>--0
3l3´4B5˜79 ’ b·vÄåÔšíJ]ó&	9	Ô	º
¨ý V Á:g
–ü]^#Š&À&'ç'š(Å(l)í*>+U+P,/!/"0I0
4E4¢4Æ4M5Ó5?6t6�6�7S8ÿ8) FÞxRDU¿£	Ä
[*MÂø­á°E¢ô0oã¯ 7"±%æ*x+…0_1w2}5Å5Ç6'7}8W9S NÔlxÄqÐ:>O¢è÷ü$ýc	ª	Þ	7
¢
½
é]ç©ž²í ZÙ)j{ò’–YM!K"T"^"h"¯$%-%_'ô(")£+Ô+ÿ+·,ç/£0Æ0ü01Â1
22:2X2K3z34f4š4ú4¾5N7È8î89‹9H  ì)Ç�-8i‹Åh	D	L	”5J…Ôšåá,˜-BƒŽ9O™¶"•¶×¤"¹"È"Î"ã"ç#i$ó$¤%ƒ'¿'ê'[)’+Ÿ,Â,Î,/c/x/)2²2`3£3A4¹45'6“6ú6x7}7„79K Ð ¨Ô\„‰¬åË(eA	«
Ñ
ÍânzNÿ¤_…:ƒ¥N„Tp‡!8%Î%*&‘&Ü'P)1*C*g*š*©*Á*Î*Ö*^+ç,E-}-æ-4.|.¬.Ù.0/3/~/È/ä/_2–23Ó3ø3ý3 5k5T7é8996  [ g ÌÝc‚-“	fÚ;èï¹ÞM” ów‡.ƒ: Ö F!V!�"£#[$å$.&m&6'i'Ç*ñ,!-.ž.¤.¾.%/0¸0Q1Þ1l4¸6Ý6) @ š j{ô@èdDXÝøð$?7½7 ©�+S!3!ò#;%Œ%&\(²(a+”.q/6Í6p7'  ½ êl³ú‚û2Ypæ÷{¿ûb~àOž!c$h%L&…*£,¬,ù,s.*/32\5•5_6o6î6·9g 5‰û bù>	&´œ¦‡QiDtå£‹žî&7X`Œ�úŒ¨J [ u „!—!Ì!Ó!ë!î!G"o"G#a#$$$$~$Æ$í$€%ó%&a&œ&½&Ð&Ý&''3)G)u)x)5*w*Ä*Ó*ê*	+H+õ+,\,`,´,Ù,;-q-‰-­-Ì-ã-..ˆ.‘.Ö.?/U/X/¾/0+12\2Ô224ú5K6h7< E ý€¥¼w“w]	„†ÍSò`À„‰e°×G®6 B f!�!Ù!æ!"X&c'>(ë):+X+‚+À+w,J-“-.“/ /Ý/ì0b2‘2¢2È24=5õ5Ô67r8Î8E Y ‚‡ögÖƒT­5x°ùŠß…ù|,cÏnáó!å#$%÷&	'Ú'g(n(®(Ö(ð(N)”)Ù)�+›+Ì,å,÷,-�-*.µ./j/Ë/182Ñ3ö3405f5Â6Û6ÿ6³7È7L n¤nHJ˜š°²Yš•Ê¾ÙX¡ÕaÎ|à#1 R ß ý d!~!•!¢!ü!")"g#¸#Ú#$®&'A'F'<(Ú(:)�)¾)×)*“*¬*F+ø+Z,-/-9-‘-Ô-V.g.Ü.[/è0ê0 11ƒ2À24q4�4Ò6D8¨8G Ô u˜5Êý	d
g
Í
¬Ž»àEÐÓøOZ›Pp¡[ÉÐ~! 3 k!þ!"Ÿ#)%Y%‘'š'N(È(K){)¬)A+½+E,X-]-N.//M/¹/Á/0@2C23H3Ö3è3ô4÷4�680 ÷ <p·9?Õ>Láqœù@î#•ÝAjîûÉ…Þ"7s£ #%l%�(Æ)Í+.n.�/“1œ1…2™2I4Z6w6¦7�8= Ú  \7<#¤éð	é	÷	 
^
$£T˜/F†ÑÁlëløÐ>m „ ª !@!-#i#
%u&Ð'²+Æ+),Ã.C/M0V1%5™5‰67"78Z8l81 Ÿ0¦ÏR…´ŠÐq¸kº7@àXÄ›%� <"º#_$°&é(T*`*ù*+K,-O-ø/0Q0ê2©3®3+4p5y58‹8M 7Sk¡´¯â`,ßåüäƒ°’?fÆ¶Ñg@£¯Ø0Ûl:=‰Ä Ü ÷ *!=!$"+"Ü#%U&¶&'9(ª(»(Ð(Ó(‘)*Q*k*Ñ+,,-….9/õ/Ú12Â2B3¾3•4±4æ46š67"8F8¸8) )•8c”j
�
Cµ
F”Q�ÛWÛ¹á2�ª…¦'ÕÃ É ò"#Ž$É$
(L+I.123s4d6]9K „ ÿ@X×:	!	˜
t{™­ÿcf¾ÚahÞÅô5R¬q}CoÃ§ç/	!!š!"²"Ü"M#´#$Ó$C%ˆ%3&Ÿ&ã&û&{'©'{(¢(ú(ü)*L*$,¾,ü.m12ˆ34]4‘4Â4Í46A7¾7›8ª8A ¬ ­MVÌÝPæ¼
±
Âÿ[HÌN_DSª5åE"Ï Ÿ !"3#’$´$Q%&&&�&((Q(Ü( )‰)¯)'*•*ú+c,1-…-Ú-÷-?.i.Ï.]/”0z4Ú7�9b YjãÂGn‘ ©ð—Çl i»|¦Ç“Ôlòz‚£šî $ § €!¶!Ö!à!3$9$§$ð$U%ƒ%æ%ê%¹&Ã&à&'C'H'W'n'–'¦'ú'(Œ(å(q)Ñ)ù)‰*®*²*Ü*þ*,,H,t,©,Ü,-`-œ-.".&.X.å./
/0K2N2+3w3…3í4ï5K7Ï7ü7æ8T ðb�ÎóJŽœ¯6Zpºf”Â}Äv§ 29=Lù@â^Ÿ´ í ½!Á!Ï!/"B"y#ß#$J%À%á%"&G&ç&‹'´'¶(¿(6)º)€*,, ,8,ß,S-³-D.Œ.¯.Ë.Q/­/«0»1/2}2å2T3Y345Œ5­5á5B6ª6´6t7O é �®N²ƒ
í
Î¦¯-6£ê ø,²uÅ?‘«½È T r!±!z"}#†#
$$o$ú$''"'¯'â'ý'a(Ë(<)€)À)Ý)*n*Œ*¹*f+o+ƒ,ë,--c-t-¿-Æ-Ï-\.™.Þ.Ä/2Œ2…4©4Û4j6&8†87 sù"f% ~
Ð/bAˆ;ˆ*—Úsö¢á ÿ ¤!'#R#�#ª$î%&g&”&»'S)ç);*++4+Ö-8.R./H/u03É45Q5×5Ò7*8V8–8Þ82 = }ªÑnu"ô½NfbPu\ëDLWòv]Šð*5†m!÷!	"#“'I*C+â,B-¥-ª-ð-².¶/»/0;055´9/ 9 I Iî*äÅ	Ü
£[h�Í¢¯Ô“©r˜$úˆÎÒ%“©þŽFE û"?#q#;,°/Ô/¡1I5Ì59Ê9  L	ÝéÊ	Ï
	l³¢y6½º K!\#¡#+%6%Ì%4'/*z.¹.¡0”2ê3@5G6ö6) Ø 3iº¤’ÙU‡¹énœðC÷zøi!»!Þ!"@"E"m""$Ñ$ë$¹(÷),-G.P.�.O/W3§3ä8%  7 Ö Ú,ÉÄ	Ú
Di¢¸§õ2ø�Ì	ü£Ë O I!Q"e" #)$r&·3-6E67­9: Ëºïïw[	‰çýcÞ.Dª%W�½òsÃ€ÇêI½T›Ðþ:ÛôÆ#+$[%f)¥)++¦/í/0~1¯1E2\3Ø3¸5,7‘9¯9Á9Í9Ö97 o 2¿.äC¶
GXmË	Q	¯	0»ñWÈñŸº8æâô¨ý?Ë&Ï2?RÒÄ¬!í#´%I(O+Þ0j2¹3 6/6N9…9•9ä96 ‹ ƒžÜá‡šv…ÌL�®ù�´¸ áGX(‚&yÕ£uÀì1g$!2"#;&œ'ð+.,T,ð/–1Ð2á46¾6<7=8O8Õ889+ N j ,£örÁûŸž½%>¯üöCÛöÍŽì;?gè6C = ‹!ˆ'})6-Z-e234Œ4g68' ›°øa•@tX	
2
,i`§Àú5}¾é; ÷• · J$c%Æ%|+w.„/,0Ö0k3°5µ587G96  ÃEwÝÏÆ,¾¢	©	“è—,ì8	˜ón® 6"S"]"g"™(!)=+/Å0û0(2J3_34™4¸4ù4L5&6s6’6&7w7|7Ç8í8V9Š9  iÓóÜckm÷>¼1zMN9 �"f*„*¨*)/}/225[5”5^6·6Ì6o79> v�’ÿ£f‚G—4ñ~sƒä ŠW+‹Í€­Îß"m0 5 A I Q Ë!Ò!ê!û! "f#Å$&Ü&@'ï(2)9)M)½)é*�+¿+,I-T/072Ð3/5ù50 [6~—ÿ	f
«>Ô—.ºÏÐø?ÏÒßk÷Ý!È¢,#^$t&M(_*W-Â.L/0B2­3ó4ö4$5x5
78�8E 6(LR þ?7i
sÞÁãB‚½EŒÙGg‘ó4QCRÐf«?®/pÚ©¥¦Ô. )!<!"³#�$‘$&%&8(P(z(ˆ)®)&*Ð+,#,„-Î.û.Ù12y4å4\9œ96 ­MÁ}
5.Fem�¥,ÿ÷u{8<r’ky�õÄí¡�# ³ À!|#¥'(Ê(**G,_-˜./G/Ã/0J2ä23ì4¬5Î73 < H 2|©¹í)ÜZgkŒ‘Me¡®²ÓØa’—C›x‡Ñ$5u’¨¼E¹yï÷"Ð$ö)H*-µ/45³9É9D 
 Š ¾Ù¹Ê®	Cðæðÿž¹Ýà-7Få $'�V§%ñrÂË%¼¿Îëû1š>¢fN P"d"ÿ"e)¤)¥/ï/•1®1i2Ï2¶3à4+7;7<8�9”9¬9À9, +h¢ÂDqú1
’�ç$ûB–+Ú0¿:>fL\"I$˜(e*ƒ*§*{+5-(/ƒ/+0'2^3‹4“5´5]677F9, 'KQý‘–l–¤-+÷>þŸÏe>­ôVÃÞH ² (!é!&Ï+,V-Á.0I2ë4«5ø5[9›9N *g¨ìù‘Bœ£æ#*ïYf‹�¸Üö6Ld ­Ò×ÿ#=ý*¦ðdÊt‘¬»$Íêó09=DÝöc"þ"H$—(õ)´/*0H2Î2µ3ß4ê4ª5³5\6*7;8E9Z9�9“9š9«9²9h fêKLM¼Âêô !"#A@A}‹�XeÚÛŸ¬!"ûü)�¡±·Äñò\.VbcÉäüýþ	
KQ^_�#³òÒ×<yz{|™šs"t"-$F$G$A&€&�&‚&(˜,™,ö.™/¨/³/;1<1G2›2€8~99€9¡9¢9£9¤9©9ª9±9Ã9Ï9Ð9F   % NÕ;´ëõñrÁØ¹	BN~‘¨óyÀ´É�’ô‘¢¸Å]/¾ÕÜåL`ÓØÙu"’#ƒ&()Ý+Y0}01J1€1¾1Ò1œ2¥2€34.7Ã7A9�9¥9Ä9Ø9< ,:ë’½Ã	$-U„h	-
õVØŒžt-t1ßÒu²óÞWÿR´u};›`!Â#.$»%÷%B&2,š,¶-÷.š/©/i0�0»0=1±1Ó4Œ78�8Ñ93 ! & ù >2Ö{G³C:‹zÁðVe-ý‚Ýæ(Ma ª"¿"Ô"é"&%X()§)*”,ô,.p.n/Z0 1¿1Ø4:5c5Ä78Û8Å9B '¡¤¸»<MÜìñöosÙ]	º	*
ÝàS÷Oã5’é$0kŽ£œõ*’ž(¿ÖÙìm—ÔÙÚY"Þ+~0�0®0K1‡2œ3 4K4¨7e8B9d9‚9¦96 oOóŒµßòy™Å}	>
Ç$8s©ÐôPCH£ªÕÙä’§÷†î"#(,)Ê0ñ0'1b1v1�1Ó1ç1¦2^8’8¿8z9Ù98 { CA©ÍÂ°C÷×�oµÊY›l“¹Æ^0àg*š v"“#M$‰$Ì$Ó%„&¦&[*Ê*+ +51Œ1¨1�2=3�3�45V5„5È5/7Z7J9v93 — â ;ËìVhG —ôÅŠÌš�±<œ¥( a!/$%n%C&|&Â(h)â*›,ô-©.ª/þ/b0‘0N1q1Ç1€2 3Ÿ3º5—6�78¥8µ8- Ú
..
öÙ<�ÅaSpª@ßX‹$�vð Ã#ø#{%(+-+h,..ø.›/¼0Ù0{1ž1Ú3»6«7/ * è-r“¾Ä%—…i	…	 ×ëxŸF7à)ËO[ µ¬¾¿È#Ñ#]%('’()·-ü-Ï/²1Ï5u8‚8&9Ò9) rz’²
Wu.êu12IÓv³S~ùB#û#W$!%¼%ø% '·'È)"*‡+3,@0j0>1Ô436y6.8r9¼97  æ ý ?å|¹ÐÔ±	µ	ô	[
¥´YÛÈ¼›Z­çfË³Þ Õö4•ˆ Ù %¨%¶%\'Ë'Y(§(¨)¶*Q+[+g/[0Â3737Å74 " ' ú å$3×ÜPÍ,	ñ	X
HTc;ŒfñøJæ	çNb�˜ÒéàmëØk#P&)È+•,ê-à0‡183¡58Æ99 µ°>%<S§ñ/	‹	Ò	Ø	5
o
1?ÀLn€°ÐWŒ.c€þ®úC)„§£ÕB!x!™"«"À"Õ"ê"�$A(A*q.o/â/!1]1À135`9I ª Hu8÷		ç	û	
b
¯
lƒÌD{¡ÂèC^¼Mcÿ·ƒ›5}Y ¥ :"K#7$'%ä%S&'ø'Õ)*Ú*Ž+C,¼,õ,M-..e.‘/O0T1k1-2Š2è2F3Ù45;5d5í5Ù6Ø7Ü8( 5 L 4®=…ítò¼ç 	È	Ü‡•á�ËöUnlL¬ Ü!p&K(E/0Ã0Ü0+6$7m7Ì769ƒ9B (0I¢§¹ÓKÝèò÷Ep(
+
<
EÞþTšä6¦!‰
Ð�n(+ApŸ¥×Ú<r˜ÕJw÷Z"…+g0Ž0ï0%191H1L1Š1;3�316©798\8C9p9§9H 3 q V‘XŠŒ¥¼ÑIæ¶Ú
	»	ë	ù	

N
`
m
ø
]�á½ÈÊ'/1“ê%1lQŽö~ºn)+í:XX‘Úß† Š"?*·+0¯0±01…1ˆ2Þ2!46é6f8e97 †N²í	^	–	Î	í	P
‰
ø	#9PV‹w€ó:¤�“j>HÀhÔÛÆ º à$™)¹+ß+U0¬2Ú2ò2‘3L4”7®78J8- T pÏ9ôó‘Æ¿óõQ±>ƒT…ÊHåÃz “ø_‡­HrÊï®!Ž'(1¸13â3¦6í7“8- X˜¿P^¶–z	%
?
ˆ%9ftªMˆIÖî¨(ŒÜÿ<#)í-1X1c1f1‚1Ý3`57ê7_8P9{9J “ÁZŽûYj�ž¨ÊØà1×ß’šv	~	B
u
�
”
ô
 &NtÈÜP{œ¡µHD„Š¤tZÉã­át` z Ä!##¦#L'ñ'($(,()()-)Ÿ+§+>,ò0	1ö8g9Ú90 ^e£Bz¸H	
ú
ž3Ñ9€ÓŒ–«Úå¼î¬%É&Ô&í&r'Ò'( (E(a)Ò,‡/ˆ0Ë0w1¤1Ô1è1§203#4O67a7À8G ô ƒÓoÜS	½	_Ñ­Ê�'—ÓnÞ%ª¶Ãcœým”1+RZ“Æ w"t#”#ï#?$N$M% %^&w&§&ä)t*Ë*++!+0+¯+1�1Ë1ž2>3‚3»3Ç3ï3‚4€5ë6º78n8z8N s x ¾Î2iÃãÅ)DØìU€3œÇ¶Ö/›_w ï@‡ÀAŸY!Œ"Y#°#C$Š$Í$Ã%&Ù&9'\*ž*´+å++,z,�-'061�1Ë2²3U4v4ÿ45u5É56‹6 607[7ß7¼8*9-90939K9S9w9‡9—9A Á Ü ŠB|€ªš¦¸0¡àš	Á	
!
H
¤^¼I�SËÏZp­
é¸áž'LIhFo › /#ÿ#y$—$¾$…&(t(ñ*},‰,–-ì.Ò0C1©15'5›5h88 ^ | DGUfëÃIZ±ß
zÉïis€øûpxäÊíuºÇÍ-ÑT+æ!õ"…$Ô%…(
*Å.50�0³0ö0l2à2%3–3<4W5…5…6Ñ8@ úðiœ¡-2;qÎH˜•_>‚Æâ e‹ÍîŽ³!’!¯"Ä"Ù"¼#0$w%=&D&«&×'â(i)›)ã*û*ò+M,œ,ÿ,l-.<.ò/c0½2 364D5»5ã6Ž7°7û89, § ã »<àíž`Â¦	û’�ÒÊ{ëŸj›i²') »$}&ê&ð)b*¤*_0È1º2	5¤58b89>9* e � ˜ ã€‰°(Kãî*5]õÌ î"a$%f%o%ÿ%J&k&Ã(¿*V,k,ª.ÿ/O1r1î1!3{5�5–7Q8< ›ÌWá‹
ŒúR‚˜i®B×hØJå=�¦k û b!ä!%~%1&l'­'�)»+Ë+Q-½-õ-L.=/ž/«/s0’0�2Æ2Ò2)3æ3ô34[4o4ó5Ž6˜6À678 8¦8¶83 0Û	©÷õ^­<¥ô2qAlŒiwžŸÌþ"!4">"ù"Ä#9&}*)++@-ù.|1Ÿ1«3¶4ã4ñ4¼6Ê6�8Å8â89* % ?¶ñ°/
óŽwb¾mÇ¤GTc�&ZYdw˜ &!ù#ž'ó)à*0,§.£/W0Š7< v Vˆãd˜/´ï˜	Ð	ï	ò
íÃ%†Ú=yKÆã««ì–V%pÓ—":#ƒ$É'r(.+ã+à/S0Ú0[1i1Ü2 33�3°3Û3à3S4ž6×6¬7¸71 fmxƒ¨ª¬®o‡T„Õ¬¦îŽ!àÂ�¹ ¼ ç ñ |%Ù%Û%Ç'î){*á+i,..œ/½0ã0ì1ì2ˆ7š7õ7:9<9k95 Í …é.s	`	†	ã	Ÿ
V¶!ØìòX_Y8Ùá'~‚Â“¶¹æ'¿ÝÀÑ â#Ý%“(+¡.ú/0¿0î2¦4Ð5Þ5ä5>7v8ƒ8×8 ³‰¿ÿ&†j	º Åu¨ÁÌ@n#[&¡*%+–/0'9Ó91 + € h”PÙ2˜ R
Ç
9ŸPZ—¼Þ•¥P\Æ�µÿh“"É#^%)'ë($-¸-Œ/Ð/×//0³1R2 6V6œ7â7÷7?8) Þ™	ÅÕ
æyG×á(*ÃâeMÄ­b� ¾ ™#Ò#¡$(0() )«+Ù+ý-01ô2û2N4	6: ÑMNŠX¶;
3J’ð¯ÔL³#ò‚Xr»ú  , � 0!S!\!" "C#X$â$"%½%²&ñ&R'f'¸'k(Î)V*Ã+4,-å0˜1#2z2¶2è5	87  Ê ²Sos{³‡Æ+±ëI2¹&´#5bzˆ©Öü3ü#è$3%É%1'#*,*.“3X4Õ45r5§5S6z6£6­6à6^7L8²8m9s9½91 E“Ñz‰m	

`v/svº„>ý(9T}úµÈa##ù%&)B)É)*ˆ+è+k0á1®2n3-446}6°6/8! [ºz…Çµ
é
ÀÆw3é ó «#Ø$<'¡'ž(µ)k+o,A0§0?1:6ò6ñ7Þ9* P ´ ¸ Æ 9xÆ«µ_;	Û	©Z0çU²E¸P»ÌÓ {!¶"à"·%™&'~'Ì'ƒ)©)�*+R+-`496 l ” @˜·Ö�žÑÕ‚	²	¶	U
r
µÌ½×þE¤{[è€¶Ì¬Ï•‡¥Ú‰ªè_ª–ôt%©%
&?)b.$0\0Œ3747D  � yæ}úHV[Œ$	2	7	p	›
À
d¦ÜÉ±øœ¿8pBi�B‰ ¨!%ˆ&]'å'4(Z(~(\+v+°,{--.0.œ.¼.á.ÿ./#/./G0Ü1ñ1ô1÷1ú1ý1Ã3d4i5Ã5Å67E ç þ £ùºõ	\
*X¸aß³o¶®ÛgÃ´ß¡Ö÷¡5AÁ8E»ÆÚ �!´!-"$ø$%ß%²'e(¨(¬(½(9*O*·*,',§,Ê,-Ê-É.h/K0 24G4O5Õ566¨6Æ7$8‰8( �Ñ4ö

.Ò¿U<@Vò3ièêî)PÂÒÙk
  ? É!a%)R,£-3Î3—48- # ( ˆ dæ%0oØÈõ"ñ�²ù/ÜT`�úOÊ!•.ì‹À#Ï#ö#U$•(–,ü/Å1g2è4Ù8Ç9Q û �E�ÝNQÎõ	*	-	Ö	å	ò	F
L
Y
rÚdJO#A³ÈåKçþ‚™ÓáJÈ{ì W £ v!w$Q&ï'ƒ(&)_)—)â)r*ï*ë-ê.30Á0ˆ1Ø2ð26393D3À3í3ƒ6ç6’7Ö7è7H8x8º8ô80 .¹ÿýð
§ÁI[gáû]|$<
cn³fÏ ù !.!ž"l#©#œ(*i+É+;/á0Ä2ä3¤4¢5æ56œ6¶7ï788 V ¥± &ARLT¨ª¬(	0	5	Œ	Ž	�	Ù	p
ªíïMÑã¸/1dÿ…¨,yòr ,!C!y!&"š"œ"u$r%�(÷(n)™-Ú/E0Ì3Á56/ ‡âç}¤s	ˆ	êô 2@'oÓÈº�‡�˜Ä2Ýê¬"Á"Ö"ë"ž$Â$O'B(ÿ)"1ä1	3ˆ4³47¢8#9a9I  a¶ëukÃ(9Q™œ7=CNT¡ç#~ò	b	Ó	6
¡
¹
¼
Ã
4ZüÁæ÷±A�f¯ûDi¡µñ•/Ö%é'ó(¢+Ó+Á,b/1^1Á1	2v2±2Å45Ò5€6ù6ƒ73 Ï §Ë?b«ä'C’	ª
Ùáçî¸yL“�X„*a¤ßRS!E!�!‹%±(B*3.r.£.p/Ç/ã/·03k46n6M D X mˆõvømSX¬…Ì†”_h½ød6¯{`àZ t Þ ("`#·#ä#$%ò%W&Ï&''b';(Õ(Ù(“)Ö)*W+�+š+Y,³,ö,.-8-p-�-â-.f.‡.’/Ü/ÿ0*12[2�214Ž4<5e5J6Ñ6Ú67? ¶
;t"8ï	è	ü	c
Ì
„³#¢E…DÀ"?÷N„Ã=ršŒ ?!";"	%(%�'Ç(S*@+D,N-\-//÷/0P0U1?2é23G3*4o5Y6v6k8< ƒ « ¬U³Ü_b“9	
—
°
¬þ`ÝÅ©¸1œäkn&<~ÃÎž !ñ"#2#L#B%T&Œ&ú&'(Ï(Ò(ù(ÿ(*½,„.8/l124”4@7Ù7a rø!I±›ÿ®‚
Í®ÿ5a~Á@|¢¨‡Ãéï+:) 1Ó±t™?¦ q!°!&#€#Þ#	$$$8$n$å%&f&¸&Â&æ& ''Š'á'ù'`(µ(p))Ü)m*Û*+e+›-.C.ä.º12.2‹2S3v3¨4È4Ú455‹5à5î5A6i6³6J7s7û7)8U8…8•8Ý8 mt!óãèÄ	É	¢qKVöº!Ý!ö!"l"#>#p#©-ï-y.¸.Ó/ 0H5/ 6 �+Èà†ãîBu	FWlvP	Z	Ù
hˆÇ·b©¼÷üÆtý9«!ì#($³%q&N+ï+Ý0·5ÿ5,6779„9Õ9 M ¯`”Î+½¡	h¼Ì7|öçòmM­ |/Ä0Õ0ú0f6%7n7% 5Z>¬eÀ|
Ý–â�ðtöªo€ì+#e#�$Ä$&(7(L(î(‡)*è*—.F/02Í7# ØÁC¸(­	kï,`‘BqšÎw4==Kx
± O"["G*z+­1Š435’5:7@ )+éJ‘Ôë³ÁéóøTq,
�U›¢å§")ŠÑŒ� °Ãð½Ûã]sÌéÖb"(˜/h0|0�01:1I1Þ4²5)7:88D9Y9}9Ž9¨9D 1£¨ºLz‹ÛÞFn˜|	)
=
ßö²47B~Ö9OŠ#j),©Øã¦Øf)X"L$Ë$Ò%¥&W(+)“,m/Œ0É0ð0&141a1u1‹1æ1<3›3€495b5Ç5Y7 8]8I9c9! Êg–
Fÿw;Eo™½ø %)!*'+†+Î/Ø0M1p13ž326ª7¤8%9q9»9' ä¦Š	ú	
4
a
n
‚ËSb¿÷¯Y»ÊB€Ÿ4ßl¢×”‡ 9"@*�/†1‰2_99 2 4 H…‹¦­ÒHJ„çì	Í	ì	'
;
O
l
÷
É".08Šv”��To*G¤9;WqgÞK¹ ‰"¸+0°01$1„1Ë788: W’—Y�ú]�¢§É×•·u	y	
$
A
�
“
ù
¾2O°S ƒ‰»sYG¬Ûàþ_ y (D(ž+¦+†/‡011e1·1/3"4ì7f9? r ó ‰{½Û¼	 
^£â(]¬úë&H–2m�ÝRwÎµÕ.š,·î*Y’EåÅ ‹"ô"Ÿ%]&€0²0õ01ß2$3Æ3;4u456ê6g8)9R9+ d ùš�:àÁ¥	Š
ùQŒ'�žIi²j Ë ®"Ã"Ø"í"º$%e%þ%¬'š)£*º+K.4C57•7¯78a84 ‡µc—n³î	—	Ï	î	ì$xôv;½Æ£F¥ª�ë k¸Ë— » !!–"9#‚$q(~+à+?-V0Z1h1Û23ß3µ4É6™7
9j9$ ˜²O1	_	â	Q
ž
Æ
WÄà'ÝÂ”À?ÁŽ’¸åÜ˜# $(/(ª+Ø+‹/.0ó2M4(   ZR ˆl	
è
°
:²ù‡¨Õ2WqÇ  á$2%È%Q'j()´)n,¦0y2­2m3’3K8Ý9: “ Å x—wÅÕªùG^�#	:	�	T
š
¿
Þ²£æÂ7T”±ˆOË^©µ"ß"÷$˜&}'d(}(N*&,É,É-þ./"/#0J0‹3F4_4N5Ô56§6 9? ‡ cŽ¸ÐnõþÇôÕ	
E
K
ÀÑô!UIà{#;hÛýS_ùÉI²Oj	 u!È!¨#Î#õ#`%î'›(%)á)q*î*Q,20Ä13ã3£4‚6î7ó8: U `Êæaj8›@Qæ"&BK	'	©
»
©öÇ†Œ— `+ð”x!œ!Á$Š%N'è'ö(m)þ)¡+À,a/Ù/12u2j4Ä4À5m6‚7¡8P C ‚ q:7Ûô’šîþ–
þRW«À?„†Ë¿!Äö¨ ®sq˜ÂÍ¯!"%#_#¶#$m$‹&Á&Î&å&'a'�'&(_(Æ(Î(þ(?+V+d+™+þ0)1¹12>2Z2�2404“4Ç4Š5@6X6u6Ð6I7ú7T8”8% Çß_ls“«Íâ EV{
ggïsJ{~©sõõk"=#²%(í(ç*î+î-Ò/Ÿ04 À�y·ÚS—{	¬	î+:}N‰×âïv¼\Ëè° N"W"a"%)*) *y+{0È01`1o1¬14a5Æ5(7~8X9|9�95 1 ò Gz„\ƒ¡Ö”x	&
:
@
’
ö
¢7\‰NRau«ù®´F£)FÝJä8"ˆ"(¥+�/†0ô0d1ƒ1¶1~5ë7Q9- c Yø™Q—À 	¤	á	Å
¯&Ã±�‘·ä1Vp±  i  !•"¹$«'.()©+×+¥0Y1g1x2Þ34È67`8i9Ü9Q † Ä _w�–`mý6?P©åøý%Aœ	€	D
•
¨
¾
Ýª¢¾…‹§ÚR^Èÿ*±“—wÁÌ Ç!"´"Þ"$#§#Í&M'`'%(õ(ý($)à) +10ý01Ã12=2Y2Š3i4‰5¿56ù7ò8N ñ ÷yƒ�–Æ[kr‚’ ªÌÕÙá
“ÿw	«	à	9
z
‘
õ
¡fQ`|³îu}»[ÊçEãôI°M"V"`"j"‡"¸$%((-()))¤+¨+Ö+í+Ž/z0¤0Ç0ó0µ14~4h9Œ9Û9“   ¡ ”•ÂÕ[\]^_Åü012345Z€�Ž��‘Ÿ©ËÑÒÓÔ23àáâãä3<=>?@›þ			ß	8
C
v
w
x
y
§
!ue^_\k|äåæ	®¯°âóXYZ[±²uv®¯ÀËa N!Å!Æ!""L"U"_"i"}"~""†"###ò'ü()#)Õ+ë+ì+è/n0y0˜0
1122;2<2d3e3g4h46C7D7O7ž7É8ï8ð8ñ8\ ¢ ì OÃÖ4;GZ‹£Øé4€d	£
"v'Ov}Éêèh]�ÜcŸ¶IE![¥*lI
\³ZÛb { O!€"##°$.%>%E%ó').)r+ ,?,¸,h-é/o0™012q2L3f3{3›4½4û4E7P7Ÿ728�8¬8Ê8÷899 í_’<Užjê	I	#†Ò|Ý.j„�J�àF\Û½š–[ "	#O#è#±$ %­%5&Õ&Ž, ,/Õ1T213B4®4¾4Ï4"6û6Q7~738A8Ë8X ß "fBRÙ9Ù5�¹E	e	�	
†
w•(KP›ÕR›ó^™â:™žd·æ+JÅ| P!�#Õ$¥%î&%'(!(F(w(¤(/)b)¼*‹+,@,¹,Ï,i-º-d/ê/p0x1¥1é1 2*2³2ø2¤3ü45,5ž5Û5(676P6”6y7…7À7ø8K  � ~ÄÇ*¤5.4Œ¤i{M	¤
É
;6Ÿ4ë  C°³"—¦m®xL]äØïc �"Ë#$j$r$ô$ý$/%?%Ê&ý&+'ë')\)s+,Ó,ï.y/ˆ/‰0š01r2M3a3q3º4F7ž89 9M - £ í P×ÈCH[`ŒÆû
>nw~Êéi:æ�-‡Ô—:P¬·#´é1·Ü¥"º"É"Ï"ä"#F%“%˜%¡&s'„'À'Ó'ô'*ô*“+Ã,&-w-Ì0¨2g3|34$4œ47b7 7ä7­8Á8X Ñ „ŠæŸÌ)pT	`‡Ëo‘(5o|L·Ä…Ž�K�†ÜU¾2[”…U!"u#O$Ü$N%¡%®%+&x&w'Ý'S(Þ(W)Ã)2*h*Ï*+1+ü+�,Ö,è,î,-Â-ÿ-5.k.ò./4/Ì1U2Ÿ2323?3È3ð3ù3¿4ü6U7»748o898 =]ÁÔÝ¾	¬
Ò
ÄÖ&/káG`g;,S„¦qˆ!
#V#@$”$š$6&¨&Ö&'›*×*"+e,†,F-~-ç-ù-Ö1þ3Ð4!5�5#68ê86 ± ©Õ–èkëfB	J	9ÒÓ{Û˜Ô"ß]«<nä› O—�•#é#ð#%9%Ï%&(&�&±)D*Ì*_+¡,1/å/C4l5ì6R77{89? õ î`“…­Vd	$Îã®}ÞOd ¥þ•Å]ì \ Ç x"P#|$²$S% &_&’&E)Q)å)u*ª*Â*+°+*-‡-Ò-}.­.Ú./É/1‘1`2—2ƒ3¼3Ô3ƒ4¯4B8Ì85 h g¿ƒ3<c*EûBeô0çxˆAˆ/Æ„; “ G!�"Z#±#\$‹$Ä%Ú&G(x(0)]*,,,‚-ë/0¥3w4-5v5Ê56z7L9T9ˆ9â9>  y à mOÚ‚f	ÛQé{ÖíVœô4_�ã;¡œ,ÁB d�#D$Î$&')c)¢)*È*Û+ò,3-.¿.&/(0¹0y1¦1Ì2³3 5•6¹6Á7½8x9˜9Ÿ9H \ ÍÏS:Ú.6ºF	”	ž	
‡
gjxLœÆºÙßS�šÈšŸ¸xa¬ç× W!¤#¦%n&7'(("(*(¥())Œ+­+µ+A,{,º,Ð,e/71F1R1Ž1+2ý45Ÿ5)617+9.91949C t #ÞdC+³
–Ää)ðI]N•·×`!ðKÑ} Q!Z!‘"Ö$æ$&/&ï&:'j'‹)Ì)Ÿ*½*æ+ý,"-j-»-Ÿ.¥.q0ß1ê1!2´2ù2V4m45Ü586Q6Œ6¡6Þ6\7†7à7ù8= àC©µ5�Y|¢Ê
¥è _J¦‘í!´ªnâŸy^i
$dðp œ ¡ !0# $$s$@%&†&þ&2(³(])t+—-A.t3566q7i8!92 X)A}›«é›¥±Çeþ/¾jáN	Â	

"
×
½Þ%‹ÄÌ¡D,˜§å0%ü%(•.z/‰/Š0Ó01219U A › ž Â Ý  |‹ÅÈõ��6‰§1E›	I
¥
<
5ìñ8q®±!ž¯¹G(MJM¯TGÙ"„"Ì#ó#k$¶$¿$õ$þ$<%Ë&ì'](u(
)ò*b+,Œ,Ç,f-í._/–0�0ª1s2ö2b3|4¬4»4*5œ5Î6G7Ÿ86   k'+§¿)¹ü¥7Ùù @T¾ÀÂÐÒÔ8J[
#ê,d ‘ 4!‚"Ô#z$˜$�%,')~,Š,Ô,ð.r/›0D1N3r3(58 EQÉßÔJ[ÇÊxü:Õü3îqvçø|7ÒµÀ !6!Ÿ!Ê"5#›#Ö#d$£$G%i%‰(*†*—*€,Ü-Ñ.t/‚0—34=4–5†67>  } ¤ ¾ ¯!HVgÎì€Äð�jã;ç*‚.VP%»È¸OÆ$2ö"†$M&.'…'Ä'Ô'õ'*)*t.42©2á2ý2&3h3}3P4X5]5p6ï6Ü7Â8Ò8< . ® î - #&DRaë²otùê2yyˆƒZQ­..ÀüUá,P¸Ýf ‰#Œ#¢&t'Á' (†(¤,­,Ä,ú,'-x-_.Æ.+/´0÷0P3†5å7¸95  _ ØI\�»îà
ü
?{jËm´ûqåÎ	Ë˜;ÎFcêç!¦"»"Ð"å"#�%”%™%Õ%õ*”+60Í02m2%4�4`6c7¡7®8I 6Š‹<Ï*úä
Œ™ÓZ–Ìp�)pèˆ€‘
~‡~ŒÖŒ•Z†�V!8!ï!H"Å"v#;$P$f$…%b&Å&ó&T'x'H)X)œ)Ð*
+I+],Š-ž-®-Ã-Þ-€.‰.Ó.@/,1Í1ú374û5i7ü8@ Sjü rqU	I¸p“6}M×R�E`æðx¿ãïô!f8\ïaœtû�K p"H#Ý$x%ô%&>&y&Ñ&Y'ß(X*x*+ -ó.0d0ñ334ê5L69M …(c?	'aµ§Lžu'n��‚´\ v …!¢"Ì"b#½#%$$­$œ%¢%¯%,&¾&Þ'T(j)6*Å*Ô*ä*ë*ý+N,�,�,µ,ï,-<-m-ä-.’.×.ç.5/v/2V2]2Õ2333¡3É3?4E5¼5=6ä6V7�7589€ Ò ûñ�¢ç.3ÍPˆ’¸Åj†5L?¤ÝNVƒŸÇ3ÎY©!‚!“!˜!Í!Ô!ì!""°"Ú"E#�#Ø#$$1$¥$Ç$î$O%W%�%è%ì%E&�&¬&´&»&Þ&' '˜'Ø'Ž(ã(ç(4)s)v)y)¸)Ä)3*i*°*ü*2+8+ó+ö+	,,6,a,r,×,Ú,é,r-Í- .	. .$.(.6.=.Z.l.//V/Y/¿/ó/š1 2¾23@3À4ý6 7f7¤7±7¼7p8™8) Gþ>aÂ§	ŽË×ó|"… âé-Ø§(. 7 C g!‰!;+G-90×1c2’2ÿ3"5>5$6ë89, F ¼¦Ÿx”ÃÅ'T5.Ók„±ù·¯½í& ^!ç!"$%~&c*¥*f,ú-.`0%2£24Ñ4‚5ö58c8s8?9K ¨ ä �½ÕÞ¿	­
CüÏ
…‡Îl™ÑanÁŠHas±Éhµ<ôœ¥…HjrÚ!W#®#5$A$•$›$¼$×&ë&'J'p'?(Ó)œ*´*Ø*Y+ƒ+x,‡,K--”-è-Ð0í0É1É2
578Ï83 =áî^xÓ
^“Ó«ò0<ìfÖJT³* å �!â!"#7&Y&©&d'ì)ñ)Þ*#+Á+”/¡/Þ/
0P2¸2»2ï4¥5ñ5Õ64  Ž ² ª÷—L]±×|Õ^y±=½P˜ÐÍ ï"$%%p% &ø&¯(×(ñ(•)Ú)*W,Ž-+./ï1(4g5m5Ã6 7´7( ÖllÔ®^‹7dðü¸!ô!7#–#ê#%Ð%&h(o(²)E*œ+l,¶.k/Ì/1s1"3-3™315�5—7É7þ70 äÓhŠÄìgC	K	I„Ó)LU™äà+¬à•!âæ#ò$
'¾'Ä(‘+Í,/æ/„092y3	4D4¡4|5M7€7R8|8E Z f ™ [ƒˆÊé�Ì:Üï#66òúþo¨åö†9œú}-o‚2!U!ñ#Z$b$ä$:%g%&)&K&l&�&h'Û'O)™*À*Í*`+¢,«,æ,ø, -«.2/ 0P1Ò3÷3Ü6í6S7è89Q †%Iƒ™¯±³™›ÉP¿CØ¢í%ÔÖb§ü c!e!–!¡!Ø!å!$}$%`&'E'=(m(F)Ž)Ø)ê)v*«*Ã*+G+[,v,
-:-ˆ-’-Ó-U.~.Û./>/Ÿ/Ê/ç0é0ë011a2‚2Ç2Ó2õ344p4ô5Á6Ó6C8§8Í85 ö o¥É�=KSp›j~ß”kYišúÏ}$  2 l ƒ © ý!h#¹#k%¯&ø*±+¼+Å+Ì+J,M.€/’1„2˜2Õ3ç3˜5ˆ6�688Y8H ”®ËáOWÖå 	Œ
zäûþZe¯Ë>eÚ^|„!æ Â È ö !"*"Û#³$%2&â&'¨'	(¡(Û(�)û)*”*ù++-0-Ù-ö-h.\/“01Á2‡3½3\4r4�4°4Ì4c6™67!8E8©8·8P ïaœÍXeiâì
oÏ–¦ÿ–ÆÙKá>]ž  S à ì þ !£!y"Q#…#©$I%T%¿%!&“&B'G'm'®'º'ü'‹(;)R)¿)Ð)æ)ˆ*‹*­* +n+,‚,Þ,--R-b-¾-Õ-W.®.Ý.¬/t0ª0M2|2*3„34„4Ñ77 hÐÎ
¼ù[mB\‰ÿ4…l!?"D"ú"[# #!$ê$*%5%Ë%3'’'¸(.*B+,:,á,A-F.O.Ž.±.N/¯/º/ 1V3¦3é3Ë5õ6ã893 Õ 1™îµ„Ë	 ­ø�³üCáxÔ1œ>Ê¢0ÊÑ#!H!1"Å#Z%:&›'H(*+-,ì/0}1D2[3×36D6½6N8Ô8M9ã98 i òÛõvÀÜž+lö_®=¦õ»õ½èy�Ÿ5B< ” ¶ 5"Ž"Å%‡'|)Y-v./12I3j34·4ø45Z5¯5r6‘6¶6Ë6v7{7Æ8U9‰9a ŸLu}€6þ	e
h
4rª-=d�F�¼D‹3FfrÑPÞ7;BQq‰jx ŠÇ ¥Í¡" 4 ¿!Ñ!ÿ!"{#²#]$Œ$$&Û&?'¤'O(y(É(1)8)L)­)¼)%*^*~*€+¾+,",F,^-ƒ-Í.ú./K/S/Â/062A2ã23¬3x4ä4ò4õ4.5w58Ž8( 	 ¡JP¸ü�Û0
å�ú,äA€$–†eÂ¡e'!Ï$d)£)‚*Î+,4-U-À.'/‚/¤/”1¿9A  :Àƒg	ô@?M|×��îWx·õ5Kcž¿þ<È¥Þ‘ï¶[-º8
CtxØ‘#E$)ô)Ü+1,µ-)0X0º0½1š2Í23´3©5[6‹7Â7™9 94 z ø &=n@·òÜRârnï¢Ud›Bk�ü'ß™ ©"¾"Ó"è"!#ˆ$*É*+ó,.o.­0§1†2J4×45U5§7‘8¾8u9y9/ á qq‘±Öê–Äé`0HR©(?ÊNZ#«¤ï ú#%m%''Ÿ'¶'‘(Á(Ç)á*.¨.z1�12x6–6º6-8´8=  ´#=ä$;Û7ðÑ	ð	k¤0>GÚÇ¬Ïb­ù—ÑèÔØ A!˜"j#%§%ã%O&Ê'÷'¦(�+Ç+B,»,.f/á/N0S1\1j1,2ç235 5Ø6274 W‰Ðñ±µ»		•	Ÿ	ê	ø		
_
ˆ
\Ç&U™à ¹m'mW… « o&>*¶+D/T0Û081G1«2Ý2�3*6#7­7[859o93 Î]d8Xi0G	ó
%M�‡ÛzL‡=‚„É› ä‹yíÈqÃ!;#¥#�'Ñ'(#(+())Ñ,W13Ü3á3_5¥67`7I ] w À Û Te™¥ê/™	
hyÈîhÄ»TÒ›ÆÂ¬ìv?Q&n !X!.#þ#„$L%Â%v&8'(s(	*/+®+³+ä+*,|,Ä.B1�1•3±3T4þ45&5t5š5Š6Ÿ6¹7Þ78m8,9/929< ¦ ß ,1pˆ—‹ÑÉí4­Aáä&»#`$}%0&I&j&é&k'Ö'Œ)ï)a*¾*ú*L,j,þ,P-k-¼-.;.�/r0í1(3Z4n45z5Ž5�6â68ú8=92 u $Ue‚§©«­¯Sòƒ…
Jl�%YœžÒ~ ="ø"Ø%Ú%Æ'È'|*â+¦.R0ë1ë2ÿ2ª3¯3R4‡7‰7ô7Œ8Ä8á8;90  Ì g8Uµåñ^ÖO–Xt»§ØádÅLÃ&þŽ ½ ’"á#Ü%ê( *+#- .ù/0¾0í2ú2Ý5ã5U6›7á7ö7G ±DnÐLy„ÆÐ´
†‰Å*rÅ¹¸	ï8"4a"ñ|�ûº`€ è ò R![!×$ç$&±&ð&0';'Í)+*U*ç+-.ä0à1"2µ2,4W45q596R6|6¢6¬6¯6ß6ñ6]7±8l9> 8¢¶´Z‹o	W·`¨/Úµ«oA¤Ù ·ºçÀAºÅÒ §!³!,"$Þ%	&‡&'±'ä'3(«(¼(>)‚)Ž*+u+,¦,¯,-z-a.›.È.à.-/Û16#8ˆ87 €/DŽ	ä	ï
-¦qÙZ±"@ú.Ç\ ”Ç(ŠÁeë V ¢ ø !”(^)h+:/û/À0Ã2ï2C3¿3–4ç4å5›6Õ7ç7G8w8¹8Ø8C Î †áêtPªã6|}£a	‡	 
¸
é3àæíóYûåìxK’@âƒ·hÃ´1ÜÞ.Rñq +!%"t$Ò+˜-¢.Æ/D0¶0ã1°2‡4²4Ñ5666"9S Tlµ	°a�
Ë
"¡ý`„§î…“*9g(0Ò0°Âãz_jm%;>ß� Ý !>!#1##Ý#ã#$$%A%ñ%V&·&ÿ&':(´(Ñ(Ô(’)*R*l*+--B.†.ö/022R3u3§45ß5²67?7r7(8j8„8 Y*d¿kâO	Ã	Ø
»¡Æ¶üæ#*#o#�$M+–.{/Ô0ù02e6Ô9" *BêŠ²À 'f•
v±8D¨¯ÂÍâ3(Ê$¤&(&+—/‹0313Ý4º9/ –~¬Gœ¦È

#
k
Ž
¾G•RŽÜvŒÍÿX-S™ºAVžf«Ö“Ä ó"\&011t4(9^9 œ´9ÿ0‡k	ßu&¼Å¢E©Ü†§·ÊÊ 1%ý%(¢*J.Š/	9L B � É i7Ú™!F"	
J
S
™
Ð¿ßÆõ"6SŸ°NNp¨i
!t!›!µ#Í#ô#$l$À$ö$_%‰%—&ä&|'í'^(c(|(Í(ý)M*p*c+˜+%,¿,È,È-ý.`/Ø/2t2Ž2/4^4’4Ã4W6l6Ï6H7 8 0 b ®6írzr~�¶Uh !”"ª'ì(*Ñ/ž0n1«1^ … Ÿ Ã ë ð ^vŒö‚•/FYŠ¨	2;	¦
u |dg[Ûb½¦Q]ºÇþH)DW°H­¿ÊÚ "|"…"³"Ý"##·$=%D%Ì&(û()ß)q+ê+g-�/00m0x0—0´1p2c3‰3}4¼4ˆ56B7�7ø718œ8«8U , œ Þ !}Æ‘AQT_Ø3œ	…
È
:=mš Qò˜9{†i¯²ß–­wKÄã è0"N#Ž#Ê#$q$Ô$ü$ÿ$’%—%4& &ü&$'*'v(£(*»*ó*Š+,�,%-v-¹-î.S2÷2p34­4Î4+5�5Ú5!666¿7ã7@8: ° Àc•ç8ÃÚ4!{ÕK\;ÄãTfë€ U#{$“$™$Û$R%&&'&Ž&'v'R(Ý(D)V)°)Â)û+d,…,Õ,í,-)-†-Á-Ñ-ø-þ-j.ñ." lN*²
bz H\úA`«æcÐ’ ()()))Š)¡)Ë)*¬+Ú+ü,2-E1ž9á9E  ßW�&(š
°Æ¨´¾(ˆûý¦½
Ö
ç	Ø¥ìŠ¿ÁÃÑÓI	+F®	#c�   "ƒ"Ó#µ$û%1(	)‹,Æ,e-@.^/•0œ011õ2s3{4«4)5
6;  ­ ® ,ÍÞ"%QºÓíâ1)xU9ÍO$E6-NÅe ¿ !5!4#ˆ#‹#š#Õ#¢$Ž%-'Ã'ÿ'ˆ((*–*,Û-^.Ð.s/2ü2O3O4Û7W R'ã
O‹ÒY·o’çÖK4ï	w}M}Õóm€›Ys!7!�!¡"Ë"D#œ#×#:$e$¤$¬$V%„%›%ç%ë%³&º&Ä&ò&S'X'—'�(æ(r)·)W*¯*7+,5,q,�-Ý-.#.'.Y.Ò.æ./u/™13>4é5<67e7£7˜83 FB~Îªñ;˜Ð4m!-r°ÈÕèÒI´óƒ¤ø¶¼ì% - ä ]!á!"­#4$#%I'o'Ò)³*Ý*	080Ï0$2O2·2î4ð5,  ZÒkÉK\ÃËHñý¼§”86ïû1!T!·!6#Y$ã$ñ$&g'½'*˜*ª,/ƒ0,3x3˜3'4 4L7ý7ç8W ÊàNhÕäÈë
y<oýdÈ=“j™ù${Óƒ   ‚ ¨ Á ë õ ! !×!„#¨$H%j%¾%á&'D'§'¹'û'(l(Š( (Ï)é)ú)‡*Š*÷*ÿ*m+Ä+,I,u,�,Ý,	--a-Ø-T./©0æ0L2{2†34Ë4—5b6‡6Ð7
8X8T ñžÏÚôKt|í´�ÿ
q»,€²k€CŠºwÝ=¼É6:‰4A¤3 µ ¾!Ð!0"C"z# $é$4%Ê%#&2'>'†'£'·(7)»)$*-*,!,9,à,E.u.�.°.Ì.J/R/®/0252â2U3Z3i345Y5®5C6q6µ6ô6u7M8Ó84  Iûp��¿•Ãä¶è+Jý/Qš¨'µ>M¹	ª×î ¨"½"Ò"ç"‡$µ'À(�*+,T-´-¬0¼1~2~3Ö4T5¨5,8³8t9¾9,  ¿ ³"cWh°[Çg<~ƒÅŠÎ&uPÇÂ!ý#K%Á%â%N&Œ'ö'*=*.A1ª2æ2”35^5s5¤6_7Ý7n9@ ~ ¥ Ë °TÏK�Å7ˆñ‚	ÄÈì3Ws$3c{%€úýã_÷"à#×%H&i&è&/'Å'Õ'**:.þ2'3Q4Y45�5â5T6{6«6®6á6ð6ó7°8Ã8à8L O{³Šn	€
·
î
2p§úë.w?„ù)-[//³ÁÆ-@Q¹ÞêU !¦!²!#~#$ð%'°'ã'=)�)�*+g+¥,®,-y-`.š.Ç.ß.,/Å/C0µ0â12¯2Q3†4~6±6Ô7æ7'8‡8 F
u°7t»þR®U~eû¶É’)#£&ø0Ü4¹9E / a ê ï ]u.ES^
„
lÏz…yývVâ¬¾É g s!{"##�#$p$û$‘%–%–&#'(b(Ì(Þ)*o*º*p+‰+—+é+u-Ç-l0w0o2�2o3.4‡5Ù556k608< ¯ Ž%¯³½!$'b~‡”Òìúa03¤wzë‰:bT#‡#Š#Ú$ú%&'u'Â'þ'‡()C)U)Á)Ê)*„,Å,ì,û,-(-d-À-Ð-].2ª4à99  gÈ&â
ê
A}ÑÍncæÏ
<,˜ÑH÷5ëã ê ô !ƒ#¬#«$š%&¼'Ÿ(¶)è)ö*6+l+p,×-S./70¨0Î03&4Ÿ4Ê4;6a6d7¢7W8—8+ ÙúJ�¾þ
BµÃÇ‰ü
r‰Í™Ü<t£§"¼"Ñ"æ"Ö%h&='¢'<*+9.I/@15S5ó6ò7+8¯8ß8) ` \t$¼]}†ù
¶
1kxdé !¥!#(#S#Ù$�%•%ï%•&T)+–+B0v02n2Ø5Ó7ß9,   #»{|Ž¼½ïðñòóôõö÷øá
ý
@|0ÌÁÂ	Ì+ÛÏÐG4èâ #‚#5+•+ž4R5  BÚ  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA   	                                    A                                                                                                                                                                                                                                                                                                        `                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                 (                                              
                                                                   
         +                             /                                             (                                          `                                                          	               	      &   6                                                                                 .   	   
   A   #   $   	            "               ~   R          
                        3                        )   $   +         *            	         =   !             
   Q   W   g   (   E   L      O   U   W   =  	   Ñ   *   ?   6   <   !   4   À   8 	                A                                                                                                                                      `                         
                                                                                                                                                                                                                                      	           (                
                       
   +           /               (              `                    	     	  & 6                           . 	 
 A # $ 	    "     ~ R    
        3        ) $ +   *    	   = !     
 Q W g ( E L  O U W =	 Ñ * ? 6 < ! 4 À 8  Ñq  ÔK  Ô§  Õ  ÝW  è  ö»ŸyÔêgg!9×%7)7)8í1¿ ÅÕÈAË€ú•µüÏ-šw<=”ýï¬¾1!s! !¨"½"Ò"##T#4$ã$#%š%×%•&³&'Œ'Å' (7)‡*¯*++-�-×-Ì.ß./®/C0™12þ2Y4â5569‰ Šè/¨µ'ß6ñ6£7F-58)|)Ë568)|)â1í�Ð 6– ½ß&Ô*78-W#®#Ó)%2Ñ4‚5¤W#®#d@V{œ(ð2ƒ6ƒ6bá%'¶(,, ,Ü,Æ-H/ø%É ¬óÖ6ÅÂ+‰1Ù273„6Â+„6x³2 ø.#7¡„ °&T#÷ MSb€÷	^
]¦ -Yµ}ÄÁ#W`vù7ÐëO¡ùqcŸ R „ ó!"}#Ü#
$$$$á%ê%X&°&''‘'¦'¶(¿(Ó(Ü(3)€)*n*w*€*Œ*Ó*	+f+½+,, ,Z,Ü,J-O-“-Æ-Ú-.I..ˆ.Ü.?/H/“1/2ƒ2™24q4p5B6Ò68[Ãõ<8%~ ¾rûS p Fwýr¥ò9Kh1ÂGJ(î0Ž3×79–98 S p ‚
œ¥KhÂî0d2Ž3×79S p ¥KhÂî0Ž3×79Fr–9Âd2ÂÐ;ðÑ	ê	\¤'&’"I&T035š5ß6ñ6ß.µß'†ãš3à%'
,,Å-„%k'í{-*°.t`=)>$Ó!Ý&Ù,²!°'¥,Ç.¸,6,=)Š.é%,!.G
Étž–$40Ð8jN	ÈÍ0Ì8¯ç0°'Ç.¸!Ì8![ÐjN	¯6è3Ò>$Ü½	?$a}a2"M Ý1�‰m	3%É%1',*	"½µ9è.ë3Â&nR	+î3C!qâ9h2Hð*ë.+|6!_._.	½µ9G&
:
£œ\ëÌ	Ñ
!Ý$¸ QÕîvm·#(°6Í
Ÿ#)%è3æ
°À¾,0Îæ
¬3p·6o!}!d&ß'-â.ÊNäý Ø-þ}ãÊ!Â(ñX¸’Åjg¤¡´!Í!Ô!-"1$¥$î$�%ß%è%E&Þ&²'½(ã(4)¸)°*ü*	,,,6,r,§,Ú, .$.É./¨6xÆÏ¢(Ò1¥2Ø9/Äv(,+5í1×„ø}!Ù'ä,-í«1‡ïÐ	=Ó—"o%i,à/[1ì1 3ô34À6J "ºí4`$I&j&¾*Œ ¯U,«²ÀÎ>™‹ )4n5²>™‹ )4n5Àâ
ÑÕæv'V)Á-70#–z	MˆÖ`5Ï4cŽ$2÷ #²>™‹ &e&+)4n5X¸-"ß%²'½(É.¨6Ý
q#ÀÓÜ½	?$É&° •\;}!‰ þßE&¾î/š {ð7M0#3:4Þ$º8¶¹Ñ Ù5	¬Ë
SÃÊ0	9ßQ }!"Ù#@'9)½)’*÷+Z/¿2¶ä9» Ç 	¸Î#Ý
æ
q¬3p²¾·ù6MyN4wŽµo!}!£"¸"Ç"Í"â"@#d&‚'ß'3+-°-â.>0P5µ9µÙ
ÐNi_%Nc¡)2-�ÏaÉ1K#Ë í"þ%j,í1Ž5•7 FÏ³á
â
»Ñ§.3zæç!¼è$3¥!Ú$–&ò&u'Õ'b(U)�)Á)�*++5+à,ì,-�-À-Ç-:.°.80á6ó6�ÿ%%Ú8\Ð'Q¶ÕJîvòr "Ê"V*t/=4è5÷ <îû.n.8=‚„Éq�'N JLl€! "C#U%æ%ê%²&¹&–'Œ(å(q)®*,4,.".&.X.å./˜1H˜°è0 1HÌ­ "%1xˆ#Åj¤Ô!1$¥$î$�%è%Þ&ã(°*ü*r,Ú, .$./B‰Ü£='¢'I/7's[-//3ÂR*ö/0�c?	ÏÅLaj¤J�‚* …!Ô!"1$¥$î$�%è%Þ&d'ã(°*Ô*ü*Á+r,Ú,ä- .$.×./É1]2%62!S, `2Ì8«÷ #²ÀÎ>™‹ &e&+)4n5ê	3³4ÚçŸ5ïÐ	=Ó—"à/[1 3ëÌ	!¸  –&b(Ç-�"‹,õ2«4)5úM ‰�¡ÿJfcpŠH˜°b¾;@ô—9×oŸÄ €!½!Ï!+"y#}#´#$$Ž$Q%U%æ%ê%"&¹&ã&÷&'–'Œ(ª(»(Ö(å(6)G)q)º)Ù)ç)®*	+H+Í+,, ,\,O-…-‰-�-Ö-.".&.N.R.X.ˆ.Ë.//?/M/Q/M0è0 12‘4É4"8&8V8 ¼$Õ':.á6<#ïÐ	/=øÓ@!—"à/M0[1i1 3×6•7¶GXŸÝ
qâËÏGRj2ä9	‰>(X+J-7®+�1{)X-^á#+Ý5â
Ñæ$�»[Êç`"z0Œ9ú_ù‹n"F#G%‰(†*€,4Àp P�6è6/µÙÄv(,+5}e
ªÑÇK/A2ò4õ4j;Ñ(2xÏ	ç	DT1ø		
_
¹… >*·\Ñ
è:š(.¢4¸6ýœ·\mëÌ	Ñ
”²è!Ý$>:Œ™‹ ¸ T$s%š(.£0²234)4¢4n5¸6î6„7˜7 QÞ)µ¿ÁìÚjN	I
×
oËo(œoãÔÎÕ
Z´î!vªÈçM dêÌ#ó#˜$&ì'c)¢)60Í0`2Ÿ5Ê5å7Ì8Ò8nò‘8ô34À6ç.:P’ Ô]"0€!U%æ%ê%¹&–'Œ(å(q)®*,.".&.X./Ÿà�=r‰$Šõ Ñ!Û&'1),S/0ä4 TÂ)Õ,þ-j.ÀÕ	Ë
Ÿ�ä4\Ñ
NÀ
ÐíÕNci_%¡)2-«1Ù 4d9Ò$>$Ý¾	„V#@$”$Ö&›*†,~-®+ "Å;'ñ6�+&î,´ `4°a•êÀ¾é• ž$|+,0	3³4t!*q9»9³ßv+ 2Ã5Å6)jN	×
ÑŸÝ
Áqû¾G�r#=$&+%0FHYÞ
rãÉtÌÐS„(40k2„6Ð8Ï³Šn	»§.3@¹¦!²!C" $é$4%Ê%2'°'ã'·(=)�)-*�*+,9,¥,®,à,-y-E.`.�.š.°.Ç.ß.,/®/U3ô6‡8jø?½7+3!Œ%q/‚ÛKœ·W#S,Sk´¯5`ý	d
g
üƒ»E’fÐOPpÑ¯l:=~! 3 Ü =!þ!"Ü#%U&¶&9(N(È(Ð(Ó(K)‘)¬)*Q*k*½+E,,-]-…./Á/õ/02@23ô478¼ È 
‹Œ¸#$²¾ù‡�­x¾w#í%U'”'@):*3+±-Ä-7.Q.Š./P5Ö5¶9&â
ã
‹ÑÒYæç	}}ÕY!7!:$e$«$„%š%Ä&ò&S'¶)6+p,�-Ý-Ò.3;6d7¢7—8¾#S$¡-è.Ö243ë3å6�7C³
Ä)·`!ðQ!Z!Ö$æ$&ï&:'Ì)æ+ß1!2´2V4586Q6¡6Þ6\7¼ È 
¸²¾ù�­x¾@)¶95ý	d
g
»EÐOPp~! 3 þ!"N(È(K)¬)½+E,]-/Á/0@23ô48Sk´¯`üƒ’fÑ¯l:=Ü =!Ü#%U&¶&9(Ð(Ó(‘)*Q*k*,-….õ/27¿ Çg~ÅuPý#K%Á%*A1”3s5Ý7å ü ¸ÏÓ°	´	ó	Z
X»šæ²Ôõ3µ%['µ*P+Z+Á37úðœ¡-2>‚ÆÍ’!¯"Ù"0$D&«&×'â(û*ò+<.ò/½2°7YjÂG»¦“î¶!ð$©,+3w3K7ü7æ8Sk´¯ 5ã`ý	d
g
üƒ»E’ÇfiiÐO£PpžÑ¯zXl:=~¨ ! 3 § Ü =!—!Ì!Ó!Ö!ë!þ!"Ü#$$§$Æ$í$%€%U&œ&¶&Ý&à&'''C'¦'ú'(9(N(È(Ð(Ó(3)K)u)x)‘)¬)ù)*Q*k*‰*þ*½+õ+,,E,H,`,t,Ù,Ü,-,-]-`-q-Ì-.…./
/U/X/¾/Á/õ/02@2K23…3ô47Ï78Ï&³Šn	â
ã
‹»ÑÒY§.æç	}}ÕY3@¹!7!¦!²!C" $:$e$«$é$4%„%š%Ê%Ä&ò&2'S'°'ã'·(=)�)¶)-*�*+6+,9,p,¥,®,à,-y-�-Ý-E.`.�.š.°.Ç.Ò.ß.,/®/3U3;6ô6d7¢7‡8—8Ú ¤é 
ÑÁë>m !-#u&²+),Ã.%5™5‰68l8© tö		æ	®
 çB]Lbþ¶š|X ¤ J#6$R&'Ô)Ù*L-d.E35ì5×7V¶Ù~¶¹æ¿Ñ Ý%ñÚô�kº¼4Aµ †'u.02i345Y5®5q6µ6u7®s·Ò³	V
d\äéI#Z'c.ë5] TeêyÈîhì!„$	*Ä.•3búA’ á9€-(Á–4Ç w�)-1 l³úb_6Í …ésÛÑ`	†	Ÿ
ìòXõ^­<¥ôáð‚ŒÂžÝ³ú0!S!4"¼#X$â$f'i)ã*M,œ,l-.¡. 3¶4D5»5Ð5Ê6ã6Ž7Å8€B'-búArzÍ3r~(Á’ ì(Ñ/ž0–4á9i õÀž®õõ�5B< ‡'|)Y-4Ì Uµñ^XØ&á#Ü%+ .ù/¾0í2Ý5ã5hÖg×ú ã!Ê+</Å2Ñ2å3ó3ò5¿6Y ‚‡5ù…ù|,nÚ'N)å,÷,-Ñ3ö3Û6gê
nc
<˜ê ô ƒ#Ÿ(è)ö*l+×-S.¨0Ê4a6W85‰ùœ‡‹î!G"a&G)	+H+\,‰-­-.ˆ.?/+1ú5h7 BÒÝÌbA¯Ô
öjŽ8XÒš•aí8 «%È&Ó&ì&q'`)Ÿ)Z*+/1£1ƒ56N679áî[^ƒˆÊÌ“þfÖ9o�!â!)&�&©&Û'O)™*À*Í*æ,«.2/¸2»2Ò3÷3ñ5S7è89ECŸƒ�pT	¥û5B|!:LŽ0qç¾[/µd¡ G!Ü$x&G(Þ(])+,,ò.ë/0ð36L9â9S^„
lz…vâ�#p$û$‘%–%#'*º*‰+u-o3Ù556; G 1{j±Ð#§¸î"-È9-ÉÝcü¿£	Ä
ïÂM”°¼0oÎ -!�"�"å$.&i'*!-ž.¤.Í/×0Þ1w2l46Ç6Ý6µ78£8$9‰ ½ÉþßE&UÁ»¾ú™M î/h2› Ý  |Å�›	<ñ8®±J"þ$u(ò*,Œ,í.ö2¬4*5œ5 Òbj8 9ÝÔ
aŸ)/16-ÝcüïM”Î -!�"�"å$.&i'*!-ž.¤.Þ1l46Ý6µ78BÌA¯öŽ8XÒš•í«%È&Ó&ì&q'`)Z*+£1ƒ5N67 ãÇii£žzX¨ § —!Ì!Ó!Ö!ë!$$§$Æ$í$€%œ&Ý&à&'''C'¦'ú'(3)u)x)ù)‰*þ*õ+,,H,`,t,Ù,Ü,-`-q-Ì-.
/U/X/¾/K2…3Ï7i Ì õÀžUµñ^®õXØõ�&5B< á#Ü%‡'|)+Y- .ù/¾0í24Ý5ã5" ' å$×ŒøNë•,Æ9R9Ù5¹E	�	
†
wK›R™™ž·¥%(!(¤(‹+@,¹,Ï,d/*2ü45ž5(6iqH_â eîw%=&ÿ,c09J[Êü75#*—*‚0—3†%Iƒ™¯±³™›ÉP¿CØ¢í%ÔÖb§ü c!e!–!¡!Ø!å!$}$%`&'E'=(m(F)Ž)Ø)ê)v*«*Ã*+G+[,v,
-:-ˆ-’-Ó-U.~.Û./>/Ÿ/Ê/ç0é0ë011a2‚2Ç2Ó2õ344p4ô5Á6Ó6C8§8Í8ÊàNhÕäÈë
y<oýdÈ=“j™ù${Óƒ   ‚ ¨ Á ë õ ! !×!„#¨$H%j%¾%á&'D'§'¹'û'(l(Š( (Ï)é)ú)‡*Š*÷*ÿ*m+Ä+,I,u,�,Ý,	--a-Ø-T./©0æ0L2{2†34Ë4—5b6‡6Ð7
8X85‰û bù>	&´œ¦‡QiDtå£‹žî&7X`Œ�úŒ¨J [ u „!—!Ì!Ó!ë!î!G"o"G#a#$$$$~$Æ$í$€%ó%&a&œ&½&Ð&Ý&''3)G)u)x)5*w*Ä*Ó*ê*	+H+õ+,\,`,´,Ù,;-q-‰-­-Ì-ã-..ˆ.‘.Ö.?/U/X/¾/0+12\2Ô224ú5K6h7±b–ßm~
�
ç
ëŒÁ¿êø– ­"Â"×"ì"8#—#�$Ÿ$%d%Ç%P'i(p(³)}+m,>--0
3l3´4B5˜76(LR þ?7i
sÞÁãB‚½EŒÙGg‘ó4QCRÐf«?®/pÚ©¥¦Ô. )!<!"³#�$‘$&%&8(P(z(ˆ)®)&*Ð+,#,„-Î.û.Ù12y4å4\9œ9n¤nHJ˜š°²Yš•Ê¾ÙX¡ÕaÎ|à#1 R ß ý d!~!•!¢!ü!")"g#¸#Ú#$®&'A'F'<(Ú(:)�)¾)×)*“*¬*F+ø+Z,-/-9-‘-Ô-V.g.Ü.[/è0ê0 11ƒ2À24q4�4Ò6D8¨8Y*d¿kâO	Ã	Ø
»¡Æ¶üæ#*#o#�$M+–.{/Ô0ù02e6Ô9F‚·HYÒn ßR	À	G
Þ
1ÂrÐÉã$©boÉüt	Ìè�ÐK†¿@HSžX#s#¯#î#>$B$x$–$½$&Ø&„(ã)s*�*ð*+y,ˆ,€-•-ë.&040Ñ0Ê1k2Ê2º3î3„6y8»8Ð8†9–9å9è Œò�$Y¹“Æk‡hº¥ñ¢øª¼Çµ!¼!Î!Õ!ß!."A"x#2$¦$ï$ù$‚%à%é%í%F&ß&'!'V'•'³'¾(ä(5)¹)ø):*±*¸*ý*3+
,,,,7,s,¨,Û,ê,-s-²-Å-Î-!.%.7.Q.[.‹.Ê.	//P/X3P5Ö5©6%8å8R'ã
O‹ÒY·o’çÖK4ï	w}M}Õóm€›Ys!7!�!¡"Ë"D#œ#×#:$e$¤$¬$V%„%›%ç%ë%³&º&Ä&ò&S'X'—'�(æ(r)·)W*¯*7+,5,q,�-Ý-.#.'.Y.Ò.æ./u/™13>4é5<67e7£7˜8 ´#=ä$;Û7ðÑ	ð	k¤0>GÚÇ¬Ïb­ù—ÑèÔØ A!˜"j#%§%ã%O&Ê'÷'¦(�+Ç+B,»,.f/á/N0S1\1j1,2ç235 5Ø627è 6(LRjŒ òþ?­M�7$»Ái
}
+5Ys¹.Fe˜Þbm�¥Áã,B‚“´½	E“PŒÚÙÿGg‘Æ÷Vku‡MhºÄó4Q{¥µ8<CRr’Ð4f«à?k¢®×ñ/Dpy�¢ÚõB©Ä¥Âíø9ˆ¡¦Ô.�ª¼Ç # ³ Û !)!<!™!µ!¼!À!Î!Õ!ß!"#"."A"±"Û"x#|#³#$2$�$‘$¦$È$Ò$ï$ù$P%‚%‡%à%é%í%&%&F&ž&µ&ß&'!'V'z'•'¥'³'(8(P(z(©(º(¾(Ê(ä(5)ˆ)®)¹)ø)*&*:*K*P*j**±*¸*ý*3+K+Ð+
,,,,,#,7,G,b,s,¨,Û,ê,-_-s-„-²-Å-Î-!.%.7.>.H.Q.[.‹.˜.Ê.Î.û./	//G/P/Ã/ô/0Ù122J2ä233A3X3y4Á4å4ì4P5¬5Ö566©6½7Î7%8š8å8\9œ9 2 4 ´ä#=HW…‰‹¦­ÐÒä$HJ„ç;Ûñ7¦±µ»ìð			Š	•	Ÿ	Í	Ñ	ê	ì	ð	ø	ú		

'
4
;
O
_
a
l
n
ˆ
÷
\k‚¤ÇÉË"&.08UŠ™0>GSbv”¿Úà Ç��÷¯Y¬¹»ÏmÊ'Tbmo*­ùBG¤9;Wq€—ŸÑè4Wgßl¢Þ×K”Ô… ‡ « ¹ Ø A!9"‰"˜"j#%§%ã%O&o&Ê'÷'¦(>*@*�+¶+¸+Ç+B,»,.D/f/�/á/0N0T0°0Û01$181G1S1\1j1„1†1,2‰2«2Ý2ç23�35 5*6Ø6#727­7Ë788[859_9o9e � ˜ ã€‰°(Kãî*5]õÌ î"a$%f%o%ÿ%J&k&Ã(¿*V,k,ª.ÿ/O1r1î1!3{5�5–7Q8¶
;t"8ï	è	ü	c
Ì
„³#¢E…DÀ"?÷N„Ã=ršŒ ?!";"	%(%�'Ç(S*@+D,N-\-//÷/0P0U1?2é23G3*4o5Y6v6k8oOóŒµßòy™Å}	>
Ç$8s©ÐôPCH£ªÕÙä’§÷†î"#(,)Ê0ñ0'1b1v1�1Ó1ç1¦2^8’8¿8z9Ù9¯ Ž%¯³½!$'b~‡”Òìúa03¤wzë‰:bT#‡#Š#Ú$ú%&'u'Â'þ'‡()C)U)Á)Ê)*„,Å,ì,û,-(-d-À-Ð-].2ª4à98 × HKÛ
£Ì¨OtT#†¡¸èêñÍïý�)D J!h!¤-0:0“2?5F6À�y·ÚS—{	¬	î+:}N‰×âïv¼\Ëè° N"W"a"%)*) *y+{0È01`1o1¬14a5Æ5(7~8X9|9�9e � ˜ 6L	(LR ãþ?E7“ÑÝz€‰‰ém	Ê	

i
Ï
	s°`ÞÁ(KãBv‚½/ElsãîvŒ³º*5]„Ù>Gg‘ý(9¢ó4QõCRÐfy«6?T}®½/pÚ©ú¥º¦µÈÔ .a Ì )!<!K!"î"##\#¡#³#a$�$‘$%+%6%f%o%Ì%ù%ÿ%&&%&J&k&4'8(P(z(Ã()B)ˆ)®)É)*&*/*¿*ˆ+Ð+è+,#,V,k,„-z.ª.¹.Î.û.ÿ/k0¡0O1r1Ù1á1î12”2®2!3n3ê3-4y4å4@5{5�546G6}6°6ö6–7/8Q8\9œ9=]ÁÔÝ¾	¬
Ò
ÄÖ&/káG`g;,S„¦qˆ!
#V#@$”$š$6&¨&Ö&'›*×*"+e,†,F-~-ç-ù-Ö1þ3Ð4!5�5#68ê8  Bl     .  8O        8OŸ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸ¶4©§~eQ<¦ËŠˆ�¦‡sð1Á‚ÿƒÒ7.8´eK¿!zŸÂÙ°83a‡ßƒÒ#Âï)°ƒ†í=óaÛUsÚ_†+€ey!;¯ñ²µv ÖT«¥ø£ÂèÆm™jâ…§)M‰e¥Ø–6¦¦€ª˜iüÖ™Ú–suY“3W�ei³3¶æ)äl¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É äM:=vqÞì¹ïQfÕÒí}P9¡«]P%›_¾Á¯hý9¸·­µPt‘T ß�XöÚ1•C›+Q�¸ñÒõFÿqÛ UE¯_rëTÔ�¡ˆeØ MÇ{²ç½E›TgK´Qõ@æ„z­u@–l~û½£ôæâÞ¶ÕAÒEPPƒ~ucÛhÆTl­F>ãÇH/ÕýýÄl�Ur½}Ë­SR†!›½áÉ@aÍÖýæúÍGëîfPR¶­¨s…mô»»�[›»±:³Fü 5„fSá{|Ç.,E*á±T¬tË¼~Ó»QnÈêÓ¬bA f/Œ	Y8Ï*çTl¦*l7¨Û3¿ðR: ©1áù£nüò7@‹r�Gãá_�Î+�|,Á`c8ÃÆé=L
Ãö°|ÔÂÖw�ƒ!«f38oö"màÕâwËHËË3C»NêÄ?Å/û#s¬;×X¾`óeÓhxÆ+ kõœBÏ]ºë�  è˜MÎzÄÁ¼£A³Ü{ÀÉ˜ï6‚è}{›Dè(pÖ¬a�:4Ç5²ñ¼Àx°2wVÏÍÌ<¸8‰žÏ WÇ?Ó{/5EdIñðn,lu;®öYb!$¯Ñ”¤
ù�*ÎØŠfQ_é&&¢j%Yë›YV®ZùWÕoIå[Ñ¿ñik¥­'iUËÍb)¥(¹Lµ¨©ßu™¾=ôôõÓ¿ÇÃNÃu[}ìgCOÛKÀpËLO(”0Ñê;bŸæ°Qp×”ãÀ¨Wpa»0@“b†‰q‡DÊ8ø†ß0«U°ï,g˜¦s×¨U×¨UN*¸&¥
¤XÔHZ¬º)–“®€&êVx‘TAAYe`åXE›|…Uá±UŸ8ÉTg‹¾9BfS‚aWæjUaÁÓÂe@}ù† \PvæN>+Õãz…Ãaƒ�5¸í„„<`Ëê;aFTþF›‰Œ6Ðî­ïlvl/æqdé<é<Zžž\n�£xw½©Q¼ÌN&ªTpU?Az.«÷~5Û¨…FRR¦†èSR	‡húã/@A› Qt˜±9ª¡éàÔøhU•f™ªxZ*’~E�‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâúä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!10Q3áŒ&«¾œÛˆÂjÝófÞí'[Žá�6JSÒg{Îlç!à-ƒŒÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1>† 3ÃpÌ:/3ø$hø<�ôöªL<ÆCÇ1L0�4ÇÅC:¨ÓºÃˆÎGJct=
¥©e*•©[>ª»ªfÒÞ�ˆ‡Â¿Xœ:€¯Ö'¼+õ‰Ã¨
ýbr9½ÐTGê±ú«þ9Z�äÛŽòv�_ë?±´(ÂLØyÀôøÿ>ûëVÉïjÆÓ£7WEë¡{¼3�xÊÉ.…×Ûƒ®>�Œ3�C	WðUIlËMËÀ®�®!»0�½m9;±‰ï?
³ºœn‰0åµ·û|·ÐÃÊè>ºÉÎ6r2©|XÐ«ŸBË«Ž¯=°ìðÑzró\ˆs¢ïëúü&Â¶°¤`óÛIô,–ýÝàö<<?Œûœ�Ë�NÉ0mû=;,-Î±ÑÁWröãlÀ�Ü¶DÛÅU¸(ì³ú¾[3|8ðÂ�®£±´ÃÎ�q­ðŽ¸ûÊ<à0øÌ>µë½ô-·ôÏ3Æ¢|
Œ|·<kì€
ô�hÐ<4uý<éÏƒ×ÏÊûÝØkÜ2Â>
+¡ªö<ûêO9V¶ç¶¦¶£¶§¸$ã1‰­4ì%‚èúŸ2vëN‚!¾óÃ^(:Ø82.=\£O£Ž23°pðÞ“"¢:£¶«ºŸ¶—µa*†¥«L=3à~Ì2Ïì
á­EÂˆðÃ#à$íÌã!œµNÉü=3ì5û|ñŒÈú†ÏÌwPó¢O¯o!ûXÎ:_N÷Ð°ÂÙ‹% ã|ÓqK>†ÌÓÔ¼óàï,mç³<<`úÜ³[,,cÇ\)0¡ºüÜ<ýlÀÞ?€±åÑvÃü1(Ð3‘€£,º>Ï«‚”�1—ëc+þŒšŠšžÛ6ÙœÓ6Ôâ©íYÝ·ê¤pÃ;
ºÓSƒÃÐóÓ ÛGWÃÐjØÓÍÂ„r3ÅÜŸÃÌm»Ï±»¬%…W
Ëàòø#
¶:+îƒ+k°ÃÏ@Ë°µŒ¾Ü7Ìóàû·¯³ŒíHÌ'@±1�ÏSHÓÏº·Ãëà±´ìÀÈÌn#À!@ÀW­Ï°>ƒ³?;ÀŒ,a¤ÎÅì5�]Â×}Œ<<2"Ã÷ãÐç4?3Ãï�Œì5‹ÒBÌãÐÂäø-âÝrÏ@É-M¾Nð0³>ð†ë°UÓ �<<ÈÒ°ü  µ/‚ËáÌ“ôÐôÃîŠØ2X»Â¹,Ôšç©ŒyWsE\ô@Ð0ÓâÌ—¤ˆÇbÊ­pãx\´SÇ�æM0@(Tž¡¿þ  ØÝ�X\_ªÜþ©Y¼¦¹esº®é
ŸÏY›âÕÞ*ZünIvFó3jo’£3™ª*¹³&•‰–Œ¹m•_Û¶V\¶6�ŠZiØérZ×Ei˜e¶ÍGÃ]µºQ^¦�V* V±8-ø–·r ‚9:*>þ8Âªß­�KÓ*£MO<zÂ£1!Fþ®Š|õÌÖcèÄ,Õl1Zö~+|qÃ8sTsgfôö7xgºÞ÷bÂ'hHÓðåÉ½Ï&°†l8cJ»ãÐôRº«Ò~»�*õló¨Ÿo%—
ÐÝ7mQ8`›„Ë4]§hsB2À±*s(ëÏH3šü�ú0p&ð¬ Ö{Ý·Üè*¢ÜÎm;©lø”Œèá*ÿ(xû¬ât®¬:(üëõ�•8TñÓM*Û[}Æ=ŠÛ�ÒÄÛºÓ%XÄ'	 ÿ[Òö–2#}´Ü¦Å¾£Ü”¼Û­‡Tô•$\* ¥´Šëx÷‡Á�Å0$fÑî.¨8‡¬JÙ—ë
=Œßz5Kó’>‹Ò�}ø¡¼ÛsJ×8vßh=÷ãÒ×G2
tº3Œ·Ü!›/B+‚$M§8Æ¼6JTVØ”xê®�*²Â[}¯Ã’ãÒ´¬¢·up†rwªü5—g‡l:Õƒ&O?†;Ûzøi¡ýÞ£Éa!ólW4Üg8>?_«	•³8gÑÊ?6Zqfãl1xl¥^>ÓZ?®‰s:¿}s†Ö½Ò«1½?t<±*¨jÜ
 «�Ë
â^m¶ÛdwqYR[F›JTæ%š�ö_[ZUYJ•TíUYUTåõaA^Ej´  
î    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /»<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12369" maxFusedRunOn="12369"><Segment range="12399-14216" color="#993366" type="standard" translated="1"/><Segment range="14217-14406" color="noColor" type="gap"/><Segment range="14407-14415" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="22" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8612-9679" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="20" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4447-5472" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6213-7007" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3702-4379" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10113-10742" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7094-7682" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3127-3379" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12088-12340" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8352-8546" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14217-14406" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5512-5686" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7868-8008" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3445-3451" color="#ffffff" type="standard"/><Segment range="3452-3469" color="#ffffff" type="standard"/><Segment name="-35" range="3470-3475" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5764-5788" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12042-12066" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3490-3511" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12369" maxFusedRunOn="12369" detectionMode="exactProteinMatch"><Segment range="12375-12392" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="3" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3397-3413" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3421-3437" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="ccdB" directionality="1" translationMW="11734.81" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2799-3104" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Plasmids containing the &lt;i&gt;ccdB&lt;/i&gt; gene cannot be propagated in standard &lt;i&gt;E. coli&lt;/i&gt; strains.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;CcdB, a bacterial toxin that poisons DNA gyrase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;ccdB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>3825558d-0fc3-4d19-82b8-74c45b2b3d77</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="5:26:30">2024.7.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  g<StrandColors><TopStrand><ColorRange range="2796..2797" colors="blue"/><ColorRange range="2798..3103" colors="black"/><ColorRange range="3104..3109" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2796..2797" colors="blue"/><ColorRange range="2798..3103" colors="black"/><ColorRange range="3104..3109" colors="blue"/></BottomStrand></StrandColors>  A´     .  8N        8NŸ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸ˜Ò¤
�ù•DDò›.* btHfšÏÀÇoþHÜ¸âÑ•.ü…ê#fÀTàÍ†|fH�¼¦ÀR´÷ÌU‡mUÏi~® Y•ä„ìZ¿ÆÊÕÚƒYR®”h[â�£i¶e«Šœ¥6%–—bXØrš˜Fªa¤KóZgjYÌdUÕfLÍ^A•¦ÌÌ"Û˜§‘°²[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&ƒ‘4èõØ MÇ{²ç½E›TgK´Qõ@æ„z­u@–l~û½£ôæâÞ¶ÕAÒEPPƒ~ucÛhÆTl­F>ãÇH/ÕýýÄl�Ur½}Ë­SR†!—`�7îËžõmQ�.ÑGÕšêµÕY°ûìö�Ó›‹zÛUIABùÕ�m£PT9²µû� ¿T`÷÷M²TQÊõ÷.µMH†n÷‡%‡7[÷›ë5¯¹™AJÚ¶ DÎ·ÒîîunnîÄêÍð Ö˜UO…íó¸±«†ÅR±Ó.ñøcNì1E»#«N±‰�˜¾0%dã<`«�Q²˜©°Þ£l<ÎükÁHèv€¤ÄO‡æ�»óÈÝ-Ê5�…|^?8®uð³9�Œlã¤ôq0+ÚÁóP[YÞv†­˜Ìhá¿Ø‰·ƒWˆ`qß-#/,Íìa8c¨xÿ¼7ì�Ì°ï]bøpuƒÍ—L-¡àLC¬¯Öq=vë®<‚ƒ¢`M4s9ë6ðN�Ïqï&c¼Ú¡õîm ¡ÃZ±†4èÐ,cÖËÆðOâÀÉÝ[?4S0òà|`â$R{<d�_ÿMì¼@Õ‘$cÇÁ¸±±ÔìJ»Ùeˆ„’¿FP�+æ@«;b(M™E¤˜š‰¨•g®meZ¹kå_U½'•oFÿÅ¥¬2–´�¥W/5ˆ¦”¢å2Ö¢§}Öfø÷ÓÓ×Nÿ;Õm÷±�?m/Ã-0<¢PÃG¨íŠ|cšÁEÃ^S�¡]Á†ìÁMŠ%Æ(ãâ|Â­VÃ¼±žb™Ï^¡W^¡U8ªàš”*‘cP]!h²è¦ZNº ›©YâEQe•ƒ•amðZW†ÅV|ã%Qœ.øå	™NU…_™©U‡O	•÷æ�pAØc˜88ø¯T/�ê†Öã¶ñƒ/¨ìI…]SøUlN&0HÛCº·½±Ù°h¿™Å“¤tó¤ñhzxYq¸<bŒUàQÞö¥Fó18˜j©QÁTüQèº¯Ü1øt×n¢5IH:š L4H&£ëŒ¼,mlEÒbÄæª‡§€Sá VUšf©àahªIù6U¿¥ÈJ¬¯xL´ÉFÕë¡êªã2[Ž:5uU}óŸL ¨¨ðmDç×º«Å	x:ÓDÍõg‹ë’$òJCßzÔ^âMóçgìî9oŠ} ã�H1”².Ÿ�íG)ðeÓ…À²7tý:ÅÇç|^Ÿ�2˜EL÷:üPPÁÐ›ÂSÁt3q:ô W>	º™/‹¬÷î„Ë¨†;- æW=5Ì>ªp¯ƒêŽª;v+«ã)¿á® eòióê†|ìóO4Ä´„ÄÁDÏ†0š®úsn#	«wÍ˜#{´�n;†<Ù)OI�ï9³œ‡€¶3üwó×8s?‡¢ã¿¼éÑÔâ.ð�ˆ´B«|¶ßÌÄ4ú€Ï,H1Ã0è¼Ïà‘£àòCÓÚ¨L=0óÅ0À2@LÓ	ê£Në#8=)�Ðô0*”J¥”ªTJ¤0Ulúªî©›Kzv"
ýbpê¿Xœ
ð¯Ö' +õ‰Èæ÷AQªÇê¯øäj?“n;ÉÚA|0c¬þÆÐ£	3aäÓãüûï­['¼4-¨NŒÜ!]®…îðÎ=ã+$lº_n¸ú>0Ì2=$E\<cÀlmU%°_-7/º>¸†ìÂvõ´äìnÆ'¼<ü*Îêqº$Ã–Ô:ßìòßC+ úë'8Ø5ÈÈ"¥ñcB®}.®:¼öÃ³ÃEèIËÍr p]Ì<‹¿¯ëð›
ØÂ‘€WÏm'Ð²[÷wƒØððþ0Wîr?.;$Á·ìôì°·:ÇG\=ËØcŒy³?rÙlVà£²Ïè:ùlpÍðãÃ
<ºŒÆÓ:=Æ·À2:ãìc(ó€Ãã0ú×®÷Ð¶ßÓ<Ï‰ð<*1òÜñ¯°: +Ò=£@ðÑ×ôó§>_?+ïw`¯pËø(®†«Øóï©<åZÛžÚšÚŽÚžà“ŒÆ&´dÓ°–£è2|ÉÛ­:†ûÏxT¡üë`àÈ¸ô8=r�>Ž8ÈÎÁÃÃzLŠˆêŽÚ®ê~Ú^Õ„ª–­0ôÏ�û04Ë?°+†µ
#Ã4�€“·3Œ†pÔX;'ðôÏ°×íóÆ3#ê?1ÜuCÎ‰>½¼‡íc8é};ßBÃ?f,”ƒ�ð[MÅ,8ú0wORóÏ€3¼±·œRÌðñƒërÌ-l°0±�p¤Â†ëópóõ³xþ lÇ—EÛðÄ£@ÌFŒ`²èø>®
RtÆ_­Œ¯ú2j*j{lÛfsL|ØsSŠ§µgvßª‘Ãì*ëL=NCÏNƒm_A«cO7
ÈÌ;r1¶ï>Æî°–\+/ƒËàŒ*Øè¯º­®Ã=,>ÂÖ2ûpPß3Ïƒì 2Þ¾Î3µ#0�ÄÆ7=M#O>êÜ#¯‚ÆÓ³#1¸� „	 =^·>Àú>Ìüï0±†�;°Ö<1w]ö0ððÈ ‹Ü;�CœÐüÏ¾3°Ö/I0#ŒC“à·‹uË=$ µ6ø=;ÀÂÌø#Â®ÁWN‚4ðó#JÃò‚‚Ô¾,#‡06OÓCÓ3º+`Ébï
ä³Rkž¦1å]ÌusÐU@ÃO‹2^�z#‹*´QÃ�árÑO7™4Á  mRz†ü@ø‚ƒcvu`qpe~«sú¥fòšå•Îê»¤*=fo‹Wxd©kñ¹%ÙwÌÌe©¾JŒÎf¨ªæÌšV&Z0å¶UnÙYrØÚv)i§c¥Ék]¦a–Û5tÖéEzš=X¨\lZÄà·âZÝÈ ‚ äè¨ûøã
«|R¶=/Lª�4U<ñë
Œ,Ä…úº)ó×3Y�£³U°ÅkÙø­ñÇáÌmQÌ!�›ÓØÝážë{Ý‹�¡#OÃ—&÷<šÂe°á�*ï�CÑJê¯Iúî<«Õ³Î v}¼–\+CtÝµDlá‚l",Ñv�¡ÍlËÄ©Ì£¯= ÌVkò7èÁÀ›Â²ƒYïvßs  ª‹s9´î¥³âR3£„«ü¡ãî³‰Òº°è£ó¬WÖ>TtáSÇM4«mm÷ö+n?KnëL”	cœ$ƒýoKÚXÈ,�öÓr›ú�rRón¶}SÒT‘p¨‚–Ð^(?­ãÞ<sÀ‘›G¸¸: â±+dF_­ü(ôB0k}èÕ/ÎHú/J5÷â†ómÍ+\áÛ} ÷ß�K]È)ÒèÌv2ßp†l½ý¬2‘6œãðÙ)Q[`RQãªº<ªË	mö¿K�JÒ²ŠÝÕÂÉÞ«ðÖ]œ2°ëV™<þïmëá¦‡÷z�%„‡Í±\Óqœàøý~¬&TzÌáŸG(üÙiÅ›�°Åá²•xø7Mhü:º%ÌêýõÎZ÷J¬ÆôxXýÐòÄª¡«p(®tK,+‰y¶Ûm‘ÝÅeImm)S˜–jwÙ}miUdm*TTmSµUeTEP/—ÔU…Uy©þÑ  
í    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /»<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="ccdB" directionality="1" translationMW="11734.81" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2798-3103" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Plasmids containing the &lt;i&gt;ccdB&lt;/i&gt; gene cannot be propagated in standard &lt;i&gt;E. coli&lt;/i&gt; strains.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;ccdB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;CcdB, a bacterial toxin that poisons DNA gyrase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3420-3436" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3396-3412" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12368" maxFusedRunOn="12368" detectionMode="exactProteinMatch"><Segment range="12374-12391" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3489-3510" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12041-12065" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5763-5787" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3444-3450" color="#ffffff" type="standard"/><Segment range="3451-3468" color="#ffffff" type="standard"/><Segment name="-35" range="3469-3474" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7867-8007" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5511-5685" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14216-14405" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8351-8545" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12087-12339" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3126-3378" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7093-7681" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10112-10741" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3701-4378" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6212-7006" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4446-5471" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8611-9678" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12368" maxFusedRunOn="12368"><Segment range="12398-14215" color="#993366" type="standard" translated="1"/><Segment range="14216-14405" color="noColor" type="gap"/><Segment range="14406-14414" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:34:58">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="2797..3102" colors="black"/></TopStrand><BottomStrand><ColorRange range="2797..3102" colors="black"/></BottomStrand></StrandColors>  A´     .  8M        8MŸ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸcJ�*wæUÊl¸¨�‰Ñ!šhw?!¿ø=#rã‹FT»ò©ü �› aSƒ6}ñ˜="<.ò› QH8nÓß1VµW=¥øb¸fW’±jÿ+WjeJºQ¡oŠ<.Œa¦Ù–®(Zr”Ø–Z]‰caÊja
©†‘/Íi�©g1‘WU™35yV›30‹nbžFÀZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
ŒšDÓ£×`�7îËžõmQ�.ÑGÕšêµÕY°ûìö�Ó›‹zÛUIABùÕ�m£PT9²µû� ¿T`÷÷M²TQÊõ÷.µMH†]‚Üw».{ÔYµFt»EThGª×T	fÀWï°kÚ?Nn-ëmT$U7çV=¶ŒeAPæÊÔcî<t‚ýQ�ÿßÜA6ÈQG+×ÜºÕ5 hb»Þ”ÝoÞo¬Ô~¾æe+jÚ�w8VßK»¹Õ¹»»«4oÀXFaU>·ÌrâÄR®JÇL»Çá�;°@ÅìŽ­:Æ$2bøÀ•“Œñ‚®uFÊb¦Ãz�°ó;ñ¯#¡Ú J“>š6ïÏ#t·(Ô~>ñxüâ¹×ÂÌæ1³Œ<n“ÑÄÀ¬?kÍAl-gyØ2¶c1£†ÿb&Þ^!�Ç|´Œ¼³4;±„áŽ <AãüRðß²70jÃ½u‹áÁÖ6]0¶‡�1b°¿YÄ,õÛ®¸ò
‰�4ÑÌç¬LÛÁ:4=Ç¼™Žóh.‡×¹´N‚‡jÆÓ 3@±Œs[/Á<‹'ulüÑLÃË�ñƒˆ‘Iìñ’|sý7²ñTVD‘�âÆÇS±*ïe–"Jý@Z@¯™¬íˆ¡6eþ’bj&¢Už¹µ•jå¯•}VôžU½ÿ–°ÊZÒv•\¼Ö"šR‹”ËZŠ�÷Y›ãßOO];ü|4ì7U·ÞÆt4ý´¼´ÀDò‰C£¶)ñŽkyN<
…w³	6(h—tL£�ˆmó
µ[òÆyŠg=z…]z…Tâ«‚jPªE�At… JË¢™i:èn¥g‰D–VU„Y·ÁhU^YóŒ•FpPX»ã”&e8 V~f¥V<&Tß˜bÀUaŽ`àPTãâ½P¾7¨\68[ŽØHCÆ¾£°Q&auOáTi±8˜Á#mêÞöÇfÁ¢þgN‘ÓÎ“Å iéáeÆàñˆ1W€QG{Ú•ÌÄâaª¥GSñD¢ê¿pÇàÓ]ºˆT`Õ% êhn€E0Ð0u ˜v�®2ð±´°I‹šªž mO†€EYViš§�…¢©'äXØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž/®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS>Âj»éÍ¸Œ&­ß6`�îÒu¸îód¥=&w¼æÎrØ8ÏñßÏ]üáÌþ‹Žü6ó§GSˆ|»ÀN6"Ñ
­òÛ|W3Ð#èb<0± ÇÃ¢ó?‚F�ƒÉOj¡0ôÃÌd<sÃ É3L|P$3ª�;¬8Œàôt¦7CÐÀªQ*–R©Q*�ÁU³ê«º¦m-éØˆ|+õ‰Ã¨
ýbp+Â¿Xœ:€¯Ö'#›ÝD~«ª¿ã�5¨þM¸ï'iðÁŽ³ûBŒ$Í‡�lO�óï¾µlžðÐ¶¡üm:3p…t^º»Ã8÷Œ¬‘²è]}¸:ãèøÃ0Èô0‘pñ�±µT–À!|´Ü¼
èúâ³	ÛÖÓ“±»žðóð«;©Æè“[Pë°gË}<®ƒë¬œã`×# Š—Å�
¹ô,º¸êóÛÏ¡'/5È�Áw0ð:.þ¿¯Âl+`k
F_=´ŸBÉoÝÞcÃÃøÁ_¹Èü¸ì“ß³Ó²ÂÜëp÷/aŽ1æÌýËdM°\U[‚ŽË? 0ëå±Ã7Ã�(ðê0kL<è÷ß Èë�±Œ£ÎŒÃë^»ßBÛLó<j'Àð`¨ÇËsÆ¾Àè ¯Hö�ÃG_ÓÎœø=|ü¯½Ý€6½Ã,#à¢º¯cÏ¾¤ó•kn{jkj;j{‚N3šÑ“NÂX.� Éó'n´è"ï<5áR‡ó­ƒƒ"ãÐàõÊ4ú8ã#;é2*#ª;j»©ûi{V¨jZ´ÃÓ>ìÀÓ,þÀ®Ô\(�0Ò>NÜÎ2À{Q`4ìŸÃÓ>Ã_·ÏÌ�¨lüÇqÕ:$úöòµŒã¥ôï} ý˜²P:7Ám7°ãèlÁÝ=KÏ> ÎòÆÞqK3ÃÆ­Ë0µ²ÀÂÆ<uÂ“
¯ÍÃÏÖÌãø°[]l?Ã�09
1‚Ë£áüú¸)IÓ~¶2¿èÉ¨©©í³m™Í1óaÍN*žÕ�Û~ªG3°«­0õ8<==:´u|=­�<Ü(G#00ì]Éü<ÆÛ¼ûºÂXUp¬¾/‚0«c¢¾è2¶»<ô°ûXËíÁC|Ï>°€Ëzû8ÎÔŒÂtÜõ4�<û«pŒ>¾NÌŒÆâ<$ õzÜûè0û3ó¼ÂÆ@\ì^ÃXðÅÜ-wØÃÃÃ ‚,?pî=sCó<>øÎÃX½$,ÀŽ0=.O‚Þ-×,ô�‚ÔÛàôï3à�n»]:ÓÃÌ�+Ê
Rø,°ŽÀÙ?ML0Îè­ƒ%‹¼+’ÍI®z˜Ç•w1ÔUÏAT>,ÉzAèŒv,ªÑG7…ËE<xÞdÓ�µIêñ _â
�ÙÕ�ÅÁ•ú­Ïê•›Êk–W;ªî�©üõ™¾-]á’¥¯Æä—daß31–¦ù*39š¢«›2iX™hÀ{–ÙUý»eeËciØ¥¦�Ž—%­tV™†[lÔ|5Ðk[¥êhõb¡q°k‚ß‰kw ‚ ƒ“¢£ïãŒ*­ñJØô½2ª4ÑTóÇ¬*0³oêè§Ï\Íf>ŒBÍVÃ¯gâ·Ç3‡1µG0†voOcw†{­ïv,"v„�?\›Üòka–Ã†4«¾=E+ª½'ë¸ò¯VÏ:�ÙöòYp­ÓvÕ³†	°ˆL³EÚv‡4!°s,§2Ž¼ôƒ1Y¯Èß£o
Êg½Û}Î€‚ª-ÌæÓº–Ï‰HÎŽ¯ò‡�ºÎ'JêÃ¢�Î±_XùQÓ…O4Ò­µ·ÜcØ­¸ý,M»­2P%ŒBp�Jõ½/ic ²7ÛMÊl[ê=ÉKÍºØqõOIREÂ¢
[Ax þ·�x|ñÌSFmâàêƒˆzÄ­‘~·ð£ÑÁ­÷£T¿9#è½(×ßŠÍ·4­s‡möƒß~=-ts §K£1ØË}Â²÷ô"°È"DÚsŒ`kÃd¥Em�IGŽªèò«,%·Úü0.=+JÊ+wWg'z¯ÃYvpÈvÃ­X2dóøc½·¯†šÝê<–6ÅsMÆsƒãõú°™Që3†}£óe§n6Ã†ÊUãàÝ5£ðêè—3«÷×8mkÝ*³Ñác÷CËª†­À 
¹Ðpq,°®%æÛm¶Gw•%´i´¥NbY©ßeõµ¥U‘´©QQµNÕU•Q@¾_QVUäV§ûF  
ì    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  /»<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="ccdB" directionality="1" translationMW="11734.81" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2797-3102" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Plasmids containing the &lt;i&gt;ccdB&lt;/i&gt; gene cannot be propagated in standard &lt;i&gt;E. coli&lt;/i&gt; strains.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;ccdB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;CcdB, a bacterial toxin that poisons DNA gyrase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3419-3435" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3395-3411" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12367" maxFusedRunOn="12367" detectionMode="exactProteinMatch"><Segment range="12373-12390" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3488-3509" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12040-12064" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5762-5786" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3443-3449" color="#ffffff" type="standard"/><Segment range="3450-3467" color="#ffffff" type="standard"/><Segment name="-35" range="3468-3473" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7866-8006" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5510-5684" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14215-14404" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8350-8544" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12086-12338" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3125-3377" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7092-7680" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10111-10740" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3700-4377" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6211-7005" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4445-5470" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8610-9677" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12367" maxFusedRunOn="12367"><Segment range="12397-14214" color="#993366" type="standard" translated="1"/><Segment range="14215-14404" color="noColor" type="gap"/><Segment range="14405-14413" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:34:52">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="2796..3101" colors="black"/></TopStrand><BottomStrand><ColorRange range="2796..3101" colors="black"/></BottomStrand></StrandColors>  =»     Ù  7         7Ÿ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸy!;¯ñ²µv ÖT«¥ø£ÂèÆm™jâ…§)M‰e¥Ø–6¦¦€ª˜iüÖ™Ú–suY“3W�ei³3¶æ)äl¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É äM:=vqÞì¹ïQfÕÒí}P9¡«]P%›_¾Á¯hý9¸·­µPt‘T ß�XöÚ1•C›+Q�¸ñÒõFÿqÛ UE¯_rëTÔ�¡ˆeØ MÇ{²ç½E›TgK´Qõ@æ„z­u@–l~û½£ôæâÞ¶ÕAÒEPPƒ~ucÛhÆTl­F>ãÇH/ÕýýÄl�Ur½}Ë­SR†!›½áÉ@aÍÖýæúÍGëîfPR¶­¨s…mô»»�[›»±:³Fü 5„fSá{|Ç.,E*á±T¬tË¼~Ó»QnÈêÓ¬bA f/Œ	Y8Ï*çTl¦*l7¨Û3¿ðR: ©1áù£nüò7@‹r�Gãá_�Î+�|,Á`c8ÃÆé=L
Ãö°|ÔÂÖw�ƒ!«f38oö"màÕâwËHËË3C»NêÄ?Å/û#s¬;×X¾`óeÓhxÆ+ kõœBÏ]ºë�  è˜MÎzÄÁ¼£A³Ü{ÀÉ˜ï6‚è}{›Dè(pÖ¬a�:4Ç5²ñ¼Àx°2wVÏÍÌ<¸8‰žÏ WÇ?Ó{/5EdIñðn,lu;®öYb!$¯Ñ”¤
ù�*ÎØŠfQ_é&&¢j%Yë›YV®ZùWÕoIå[Ñ¿ñik¥­'iUËÍb)¥(¹Lµ¨©ßu™¾=ôôõÓ¿ÇÃNÃu[}ìgCOÛKÀpËLO(”0Ñê;bŸæ°Qp×”ãÀ¨Wpa»0@“b†‰q‡DÊ8ø†ß0«U°ï,g˜¦s×¨U×¨UN*¸&¥
¤XÔHZ¬º)–“®€&êVx‘TAAYe`åXE›|…Uá±UŸ8ÉTg‹¾9BfS‚aWæjUaÁÓÂe@}ù† \PvæN>+Õãz…Ãaƒ�5¸í„„<`Ëê;aFTþF›‰Œ6Ðî­ïlvl/æqdé<é<Zžž\n�£xw½©Q¼ÌN&ªTpU?Az.«÷~5Û¨…FRR¦†èSR	‡húã/@A› Qt˜±9ª¡éàÔøhU•f™ªxZ*’~E�‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâúä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!10Q3áŒ&«¾œÛˆÂjÝófÞí'[Žá�6JSÒg{Îlç!à-ƒŒÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1>† 3ÃpÌ:/3ø$hø<�ôöªL<ÆCÇ1L0�4ÇÅC:¨ÓºÃˆÎGJct=
¥©e*•©[>ª»ªfÒÞ�ˆ‡Â¿Xœ:€¯Ö'¼+õ‰Ã¨
ýbr9½ÐTGê±ú«þ9Z�äÛŽòv�_ë?±´(ÂLØyÀôøÿ>ûëVÉïjÆÓ£7WEë¡{¼3�xÊÉ.…×Ûƒ®>�Œ3�C	WðUIlËMËÀ®�®!»0�½m9;±‰ï?
³ºœn‰0åµ·û|·ÐÃÊè>ºÉÎ6r2©|XÐ«ŸBË«Ž¯=°ìðÑzró\ˆs¢ïëúü&Â¶°¤`óÛIô,–ýÝàö<<?Œûœ�Ë�NÉ0mû=;,-Î±ÑÁWröãlÀ�Ü¶DÛÅU¸(ì³ú¾[3|8ðÂ�®£±´ÃÎ�q­ðŽ¸ûÊ<à0øÌ>µë½ô-·ôÏ3Æ¢|
Œ|·<kì€
ô�hÐ<4uý<éÏƒ×ÏÊûÝØkÜ2Â>
+¡ªö<ûêO9V¶ç¶¦¶£¶§¸$ã1‰­4ì%‚èúŸ2vëN‚!¾óÃ^(:Ø82.=\£O£Ž23°pðÞ“"¢:£¶«ºŸ¶—µa*†¥«L=3à~Ì2Ïì
á­EÂˆðÃ#à$íÌã!œµNÉü=3ì5û|ñŒÈú†ÏÌwPó¢O¯o!ûXÎ:_N÷Ð°ÂÙ‹% ã|ÓqK>†ÌÓÔ¼óàï,mç³<<`úÜ³[,,cÇ\)0¡ºüÜ<ýlÀÞ?€±åÑvÃü1(Ð3‘€£,º>Ï«‚”�1—ëc+þŒšŠšžÛ6ÙœÓ6Ôâ©íYÝ·ê¤pÃ;
ºÓSƒÃÐóÓ ÛGWÃÐjØÓÍÂ„r3ÅÜŸÃÌm»Ï±»¬%…W
Ëàòø#
¶:+îƒ+k°ÃÏ@Ë°µŒ¾Ü7Ìóàû·¯³ŒíHÌ'@±1�ÏSHÓÏº·Ãëà±´ìÀÈÌn#À!@ÀW­Ï°>ƒ³?;ÀŒ,a¤ÎÅì5�]Â×}Œ<<2"Ã÷ãÐç4?3Ãï�Œì5‹ÒBÌãÐÂäø-âÝrÏ@É-M¾Nð0³>ð†ë°UÓ �<<ÈÒ°ü  µ/‚ËáÌ“ôÐôÃîŠØ2X»Â¹,Ôšç©ŒyWsE\ô@Ð0ÓâÌ—¤ˆÇbÊ­pãx\´SÇ�æM0@(Tž¡¿þ  ØÝ�X\_ªÜþ©Y¼¦¹esº®é
ŸÏY›âÕÞ*ZünIvFó3jo’£3™ª*¹³&•‰–Œ¹m•_Û¶V\¶6�ŠZiØérZ×Ei˜e¶ÍGÃ]µºQ^¦�V* V±8-ø–·r ‚9:*>þ8Âªß­�KÓ*£MO<zÂ£1!Fþ®Š|õÌÖcèÄ,Õl1Zö~+|qÃ8sTsgfôö7xgºÞ÷bÂ'hHÓðåÉ½Ï&°†l8cJ»ãÐôRº«Ò~»�*õló¨Ÿo%—
ÐÝ7mQ8`›„Ë4]§hsB2À±*s(ëÏH3šü�ú0p&ð¬ Ö{Ý·Üè*¢ÜÎm;©lø”Œèá*ÿ(xû¬ât®¬:(üëõ�•8TñÓM*Û[}Æ=ŠÛ�ÒÄÛºÓ%XÄ'	 ÿ[Òö–2#}´Ü¦Å¾£Ü”¼Û­‡Tô•$\* ¥´Šëx÷‡Á�Å0$fÑî.¨8‡¬JÙ—ë
=Œßz5Kó’>‹Ò�}ø¡¼ÛsJ×8vßh=÷ãÒ×G2
tº3Œ·Ü!›/B+‚$M§8Æ¼6JTVØ”xê®�*²Â[}¯Ã’ãÒ´¬¢·up†rwªü5—g‡l:Õƒ&O?†;Ûzøi¡ýÞ£Éa!ólW4Üg8>?_«	•³8gÑÊ?6Zqfãl1xl¥^>ÓZ?®‰s:¿}s†Ö½Ò«1½?t<±*¨jÜ
 «�Ë
â^m¶ÛdwqYR[F›JTæ%š�ö_[ZUYJ•TíUYUTåõaA^Ej´  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,Ë<?xml version="1.0"?><Features recycledIDs="23" nextValidID="24"><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3113-3129" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3089-3105" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12061" maxFusedRunOn="12061" detectionMode="exactProteinMatch"><Segment range="12067-12084" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3182-3203" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11734-11758" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5456-5480" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3137-3143" color="#ffffff" type="standard"/><Segment range="3144-3161" color="#ffffff" type="standard"/><Segment name="-35" range="3162-3167" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7560-7700" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5204-5378" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="13909-14098" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8044-8238" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11780-12032" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2819-3071" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6786-7374" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9805-10434" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3394-4071" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5905-6699" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4139-5164" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8304-9371" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12061" maxFusedRunOn="12061"><Segment range="12091-13908" color="#993366" type="standard" translated="1"/><Segment range="13909-14098" color="noColor" type="gap"/><Segment range="14099-14107" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:34:43">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ?2     ô  7‡         7‡Ÿ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸ92¥zS Ÿ°oy'’ôˆ ZãFoMÒ<ìK‘ñ;¯ñ²µv ÖT«¥ø£ÂèÆm™jâ…§)M‰e¥Ø–6¦¦€ª˜iüÖ™Ú–suY“3W�ei³3¶æ)äl¬–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É äM:=vqÞì¹ïQfÕÒí}P9¡«]P%›_¾Á¯hý9¸·­µPt‘T ß�XöÚ1•C›+Q�¸ñÒõFÿqÛ UE¯_rëTÔ�¡ˆeØ MÇ{²ç½E›TgK´Qõ@æ„z­u@–l~û½£ôæâÞ¶ÕAÒEPPƒ~ucÛhÆTl­F>ãÇH/ÕýýÄl�Ur½}Ë­SR†!›½áÉ@aÍÖýæúÍGëîfPR¶­¨s…mô»»�[›»±:³Fü 5„fSá{|Ç.,E*á±T¬tË¼~Ó»QnÈêÓ¬bA f/Œ	Y8Ï*çTl¦*l7¨Û3¿ðR: ©1áù£nüò7@‹r�Gãá_�Î+�|,Á`c8ÃÆé=L
Ãö°|ÔÂÖw�ƒ!«f38oö"màÕâwËHËË3C»NêÄ?Å/û#s¬;×X¾`óeÓhxÆ+ kõœBÏ]ºë�  è˜MÎzÄÁ¼£A³Ü{ÀÉ˜ï6‚è}{›Dè(pÖ¬a�:4Ç5²ñ¼Àx°2wVÏÍÌ<¸8‰žÏ WÇ?Ó{/5EdIñðn,lu;®öYb!$¯Ñ”¤
ù�*ÎØŠfQ_é&&¢j%Yë›YV®ZùWÕoIå[Ñ¿ñik¥­'iUËÍb)¥(¹Lµ¨©ßu™¾=ôôõÓ¿ÇÃNÃu[}ìgCOÛKÀpËLO(”0Ñê;bŸæ°Qp×”ãÀ¨Wpa»0@“b†‰q‡DÊ8ø†ß0«U°ï,g˜¦s×¨U×¨UN*¸&¥
¤XÔHZ¬º)–“®€&êVx‘TAAYe`åXE›|…Uá±UŸ8ÉTg‹¾9BfS‚aWæjUaÁÓÂe@}ù† \PvæN>+Õãz…Ãaƒ�5¸í„„<`Ëê;aFTþF›‰Œ6Ðî­ïlvl/æqdé<é<Zžž\n�£xw½©Q¼ÌN&ªTpU?Az.«÷~5Û¨…FRR¦†èSR	‡húã/@A› Qt˜±9ª¡éàÔøhU•f™ªxZ*’~E�‡Õoér«+Þ-òEQµAºèFúª¸Ì–ãŽ�]U_|çÓ**<Q9õîªñB^´Ñ3F}Yâúä‰<’�÷Þµ¸“|ùÙû;Ž[âŸH8ãÒe,‹§À {QÆJ|C4ÂÀáp ¬�Ý?N�1qùß„gçL„æS=Î¿T0t&ð”Ã@°]LÜ@N½ Ï‚n¦KâÀ«=û‡¡2Æª!ŽË@ù�UÏMsªœ+àú£ƒêŽÝŠêøÊoøkˆ|š|ú„„áŸ;<ÓÅÇÄÍ1-!10Q3áŒ&«¾œÛˆÂjÝófÞí'[Žá�6JSÒg{Îlç!à-ƒŒÿüõßÎÏáÀè¸ïÃo:tu8‡Ë¼ãb-ªß-·Ås1>† 3ÃpÌ:/3ø$hø<�ôöªL<ÆCÇ1L0�4ÇÅC:¨ÓºÃˆÎGJct=
¥©e*•©[>ª»ªfÒÞ�ˆ‡Â¿Xœ:€¯Ö'¼+õ‰Ã¨
ýbr9½ÐTGê±ú«þ9Z�äÛŽòv�_ë?±´(ÂLØyÀôøÿ>ûëVÉïjÆÓ£7WEë¡{¼3�xÊÉ.…×Ûƒ®>�Œ3�C	WðUIlËMËÀ®�®!»0�½m9;±‰ï?
³ºœn‰0åµ·û|·ÐÃÊè>ºÉÎ6r2©|XÐ«ŸBË«Ž¯=°ìðÑzró\ˆs¢ïëúü&Â¶°¤`óÛIô,–ýÝàö<<?Œûœ�Ë�NÉ0mû=;,-Î±ÑÁWröãlÀ�Ü¶DÛÅU¸(ì³ú¾[3|8ðÂ�®£±´ÃÎ�q­ðŽ¸ûÊ<à0øÌ>µë½ô-·ôÏ3Æ¢|
Œ|·<kì€
ô�hÐ<4uý<éÏƒ×ÏÊûÝØkÜ2Â>
+¡ªö<ûêO9V¶ç¶¦¶£¶§¸$ã1‰­4ì%‚èúŸ2vëN‚!¾óÃ^(:Ø82.=\£O£Ž23°pðÞ“"¢:£¶«ºŸ¶—µa*†¥«L=3à~Ì2Ïì
á­EÂˆðÃ#à$íÌã!œµNÉü=3ì5û|ñŒÈú†ÏÌwPó¢O¯o!ûXÎ:_N÷Ð°ÂÙ‹% ã|ÓqK>†ÌÓÔ¼óàï,mç³<<`úÜ³[,,cÇ\)0¡ºüÜ<ýlÀÞ?€±åÑvÃü1(Ð3‘€£,º>Ï«‚”�1—ëc+þŒšŠšžÛ6ÙœÓ6Ôâ©íYÝ·ê¤pÃ;
ºÓSƒÃÐóÓ ÛGWÃÐjØÓÍÂ„r3ÅÜŸÃÌm»Ï±»¬%…W
Ëàòø#
¶:+îƒ+k°ÃÏ@Ë°µŒ¾Ü7Ìóàû·¯³ŒíHÌ'@±1�ÏSHÓÏº·Ãëà±´ìÀÈÌn#À!@ÀW­Ï°>ƒ³?;ÀŒ,a¤ÎÅì5�]Â×}Œ<<2"Ã÷ãÐç4?3Ãï�Œì5‹ÒBÌãÐÂäø-âÝrÏ@É-M¾Nð0³>ð†ë°UÓ �<<ÈÒ°ü  µ/‚ËáÌ“ôÐôÃîŠØ2X»Â¹,Ôšç©ŒyWsE\ô@Ð0ÓâÌ—¤ˆÇbÊ­pãx\´SÇ�æM0@(Tž¡¿þ  ØÝ�X\_ªÜþ©Y¼¦¹esº®é
ŸÏY›âÕÞ*ZünIvFó3jo’£3™ª*¹³&•‰–Œ¹m•_Û¶V\¶6�ŠZiØérZ×Ei˜e¶ÍGÃ]µºQ^¦�V* V±8-ø–·r ‚9:*>þ8Âªß­�KÓ*£MO<zÂ£1!Fþ®Š|õÌÖcèÄ,Õl1Zö~+|qÃ8sTsgfôö7xgºÞ÷bÂ'hHÓðåÉ½Ï&°†l8cJ»ãÐôRº«Ò~»�*õló¨Ÿo%—
ÐÝ7mQ8`›„Ë4]§hsB2À±*s(ëÏH3šü�ú0p&ð¬ Ö{Ý·Üè*¢ÜÎm;©lø”Œèá*ÿ(xû¬ât®¬:(üëõ�•8TñÓM*Û[}Æ=ŠÛ�ÒÄÛºÓ%XÄ'	 ÿ[Òö–2#}´Ü¦Å¾£Ü”¼Û­‡Tô•$\* ¥´Šëx÷‡Á�Å0$fÑî.¨8‡¬JÙ—ë
=Œßz5Kó’>‹Ò�}ø¡¼ÛsJ×8vßh=÷ãÒ×G2
tº3Œ·Ü!›/B+‚$M§8Æ¼6JTVØ”xê®�*²Â[}¯Ã’ãÒ´¬¢·up†rwªü5—g‡l:Õƒ&O?†;Ûzøi¡ýÞ£Éa!ólW4Üg8>?_«	•³8gÑÊ?6Zqfãl1xl¥^>ÓZ?®‰s:¿}s†Ö½Ò«1½?t<±*¨jÜ
 «�Ë
â^m¶ÛdwqYR[F›JTæ%š�ö_[ZUYJ•TíUYUTåõaA^Ej´  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -s<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2797-2910" color="#ccffcc" type="standard"/></Feature><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3221-3237" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3197-3213" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12169" maxFusedRunOn="12169" detectionMode="exactProteinMatch"><Segment range="12175-12192" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3290-3311" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11842-11866" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5564-5588" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3245-3251" color="#ffffff" type="standard"/><Segment range="3252-3269" color="#ffffff" type="standard"/><Segment name="-35" range="3270-3275" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7668-7808" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5312-5486" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14017-14206" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8152-8346" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11888-12140" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2927-3179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6894-7482" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9913-10542" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3502-4179" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6013-6807" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4247-5272" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8412-9479" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12169" maxFusedRunOn="12169"><Segment range="12199-14016" color="#993366" type="standard" translated="1"/><Segment range="14017-14206" color="noColor" type="gap"/><Segment range="14207-14215" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:34:25">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="2796..2909" colors="black"/></TopStrand><BottomStrand><ColorRange range="2796..2909" colors="black"/></BottomStrand></StrandColors>  ?0     ó  7�         7�Ÿ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸ92¥zS Ÿ°oy/H‚®4fôÝ ÃÎÄ¹?±jÿ+WjeJºQ¡oŠ<.Œa¦Ù–®(Zr”Ø–Z]‰caÊja
©†‘/Íi�©g1‘WU™35yV›30‹nbžFÀZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
ŒšDÓ£×`�7îËžõmQ�.ÑGÕšêµÕY°ûìö�Ó›‹zÛUIABùÕ�m£PT9²µû� ¿T`÷÷M²TQÊõ÷.µMH†]‚Üw».{ÔYµFt»EThGª×T	fÀWï°kÚ?Nn-ëmT$U7çV=¶ŒeAPæÊÔcî<t‚ýQ�ÿßÜA6ÈQG+×ÜºÕ5 hb»Þ”ÝoÞo¬Ô~¾æe+jÚ�w8VßK»¹Õ¹»»«4oÀXFaU>·ÌrâÄR®JÇL»Çá�;°@ÅìŽ­:Æ$2bøÀ•“Œñ‚®uFÊb¦Ãz�°ó;ñ¯#¡Ú J“>š6ïÏ#t·(Ô~>ñxüâ¹×ÂÌæ1³Œ<n“ÑÄÀ¬?kÍAl-gyØ2¶c1£†ÿb&Þ^!�Ç|´Œ¼³4;±„áŽ <AãüRðß²70jÃ½u‹áÁÖ6]0¶‡�1b°¿YÄ,õÛ®¸ò
‰�4ÑÌç¬LÛÁ:4=Ç¼™Žóh.‡×¹´N‚‡jÆÓ 3@±Œs[/Á<‹'ulüÑLÃË�ñƒˆ‘Iìñ’|sý7²ñTVD‘�âÆÇS±*ïe–"Jý@Z@¯™¬íˆ¡6eþ’bj&¢Už¹µ•jå¯•}VôžU½ÿ–°ÊZÒv•\¼Ö"šR‹”ËZŠ�÷Y›ãßOO];ü|4ì7U·ÞÆt4ý´¼´ÀDò‰C£¶)ñŽkyN<
…w³	6(h—tL£�ˆmó
µ[òÆyŠg=z…]z…Tâ«‚jPªE�At… JË¢™i:èn¥g‰D–VU„Y·ÁhU^YóŒ•FpPX»ã”&e8 V~f¥V<&Tß˜bÀUaŽ`àPTãâ½P¾7¨\68[ŽØHCÆ¾£°Q&auOáTi±8˜Á#mêÞöÇfÁ¢þgN‘ÓÎ“Å iéáeÆàñˆ1W€QG{Ú•ÌÄâaª¥GSñD¢ê¿pÇàÓ]ºˆT`Õ% êhn€E0Ð0u ˜v�®2ð±´°I‹šªž mO†€EYViš§�…¢©'äXØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž/®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS>Âj»éÍ¸Œ&­ß6`�îÒu¸îód¥=&w¼æÎrØ8ÏñßÏ]üáÌþ‹Žü6ó§GSˆ|»ÀN6"Ñ
­òÛ|W3Ð#èb<0± ÇÃ¢ó?‚F�ƒÉOj¡0ôÃÌd<sÃ É3L|P$3ª�;¬8Œàôt¦7CÐÀªQ*–R©Q*�ÁU³ê«º¦m-éØˆ|+õ‰Ã¨
ýbp+Â¿Xœ:€¯Ö'#›ÝD~«ª¿ã�5¨þM¸ï'iðÁŽ³ûBŒ$Í‡�lO�óï¾µlžðÐ¶¡üm:3p…t^º»Ã8÷Œ¬‘²è]}¸:ãèøÃ0Èô0‘pñ�±µT–À!|´Ü¼
èúâ³	ÛÖÓ“±»žðóð«;©Æè“[Pë°gË}<®ƒë¬œã`×# Š—Å�
¹ô,º¸êóÛÏ¡'/5È�Áw0ð:.þ¿¯Âl+`k
F_=´ŸBÉoÝÞcÃÃøÁ_¹Èü¸ì“ß³Ó²ÂÜëp÷/aŽ1æÌýËdM°\U[‚ŽË? 0ëå±Ã7Ã�(ðê0kL<è÷ß Èë�±Œ£ÎŒÃë^»ßBÛLó<j'Àð`¨ÇËsÆ¾Àè ¯Hö�ÃG_ÓÎœø=|ü¯½Ý€6½Ã,#à¢º¯cÏ¾¤ó•kn{jkj;j{‚N3šÑ“NÂX.� Éó'n´è"ï<5áR‡ó­ƒƒ"ãÐàõÊ4ú8ã#;é2*#ª;j»©ûi{V¨jZ´ÃÓ>ìÀÓ,þÀ®Ô\(�0Ò>NÜÎ2À{Q`4ìŸÃÓ>Ã_·ÏÌ�¨lüÇqÕ:$úöòµŒã¥ôï} ý˜²P:7Ám7°ãèlÁÝ=KÏ> ÎòÆÞqK3ÃÆ­Ë0µ²ÀÂÆ<uÂ“
¯ÍÃÏÖÌãø°[]l?Ã�09
1‚Ë£áüú¸)IÓ~¶2¿èÉ¨©©í³m™Í1óaÍN*žÕ�Û~ªG3°«­0õ8<==:´u|=­�<Ü(G#00ì]Éü<ÆÛ¼ûºÂXUp¬¾/‚0«c¢¾è2¶»<ô°ûXËíÁC|Ï>°€Ëzû8ÎÔŒÂtÜõ4�<û«pŒ>¾NÌŒÆâ<$ õzÜûè0û3ó¼ÂÆ@\ì^ÃXðÅÜ-wØÃÃÃ ‚,?pî=sCó<>øÎÃX½$,ÀŽ0=.O‚Þ-×,ô�‚ÔÛàôï3à�n»]:ÓÃÌ�+Ê
Rø,°ŽÀÙ?ML0Îè­ƒ%‹¼+’ÍI®z˜Ç•w1ÔUÏAT>,ÉzAèŒv,ªÑG7…ËE<xÞdÓ�µIêñ _â
�ÙÕ�ÅÁ•ú­Ïê•›Êk–W;ªî�©üõ™¾-]á’¥¯Æä—daß31–¦ù*39š¢«›2iX™hÀ{–ÙUý»eeËciØ¥¦�Ž—%­tV™†[lÔ|5Ðk[¥êhõb¡q°k‚ß‰kw ‚ ƒ“¢£ïãŒ*­ñJØô½2ª4ÑTóÇ¬*0³oêè§Ï\Íf>ŒBÍVÃ¯gâ·Ç3‡1µG0†voOcw†{­ïv,"v„�?\›Üòka–Ã†4«¾=E+ª½'ë¸ò¯VÏ:�ÙöòYp­ÓvÕ³†	°ˆL³EÚv‡4!°s,§2Ž¼ôƒ1Y¯Èß£o
Êg½Û}Î€‚ª-ÌæÓº–Ï‰HÎŽ¯ò‡�ºÎ'JêÃ¢�Î±_XùQÓ…O4Ò­µ·ÜcØ­¸ý,M»­2P%ŒBp�Jõ½/ic ²7ÛMÊl[ê=ÉKÍºØqõOIREÂ¢
[Ax þ·�x|ñÌSFmâàêƒˆzÄ­‘~·ð£ÑÁ­÷£T¿9#è½(×ßŠÍ·4­s‡möƒß~=-ts §K£1ØË}Â²÷ô"°È"DÚsŒ`kÃd¥Em�IGŽªèò«,%·Úü0.=+JÊ+wWg'z¯ÃYvpÈvÃ­X2dóøc½·¯†šÝê<–6ÅsMÆsƒãõú°™Që3†}£óe§n6Ã†ÊUãàÝ5£ðêè—3«÷×8mkÝ*³Ñác÷CËª†­À 
¹Ðpq,°®%æÛm¶Gw•%´i´¥NbY©ßeõµ¥U‘´©QQµNÕU•Q@¾_QVUäV§ûF  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  -s<?xml version="1.0"?><Features nextValidID="24"><Feature recentID="23" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2797-2904" color="#ccffcc" type="standard"/></Feature><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3215-3231" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3191-3207" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12163" maxFusedRunOn="12163" detectionMode="exactProteinMatch"><Segment range="12169-12186" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3284-3305" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11836-11860" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5558-5582" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3239-3245" color="#ffffff" type="standard"/><Segment range="3246-3263" color="#ffffff" type="standard"/><Segment name="-35" range="3264-3269" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7662-7802" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5306-5480" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14011-14200" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8146-8340" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11882-12134" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2921-3173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6888-7476" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9907-10536" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3496-4173" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6007-6801" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4241-5266" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8406-9473" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12163" maxFusedRunOn="12163"><Segment range="12193-14010" color="#993366" type="standard" translated="1"/><Segment range="14011-14200" color="noColor" type="gap"/><Segment range="14201-14209" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  A<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:34:3">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   ¯<StrandColors><TopStrand><ColorRange range="2796..2903" colors="black"/></TopStrand><BottomStrand><ColorRange range="2796..2903" colors="black"/></BottomStrand></StrandColors>  =©      Ø  7         7Ÿ`µÿÈ®ïV…ëé™P.ŒPd€hŒ¶þœ´�·moz:…‚�rëª½Æ;ì€€Û¨Þ,4CnµÆ«êlÖ6¢A=úú¯yºÝeHtdà5�oB¯iþ¢…Ríhþ ºá&êª�$u",Ž÷Ø¡Å®º-¡,”G¸˜^+=.§Jâ�¾æ¡n¡ïaNW…qÖá½NÔã¹6³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pô�ÔèØøÒ4È#þîDe„Ú6.R™Ui÷fª¨·z*�*nn¦vf¥zžçY–æIçYfÒ*‘Ù•…Ò‘Ø.ThIªtçJ¦ê¤ˆØŠïªª�Y:ß™–zÛÚ¦’vö¤
�)`ª™Zª’væ©®j“îV”Uå^æ©*ªjYjFeYU•!åV•ÕŸêQZUW”Ú«¢¬IcÓâ•Ï,q.\õôÅôÓ,ËÔÅ4pÜ6î,ãîþìD¯;ß(Žó‹½£ Á0ÑÈOÃC¾ûîÜ79À¾¯ß;ë:¿¯³óÉedwÿ½ßî¯õû"ŠÁ3wwuônÿÕ½ü°ß¼ëPœó²ûÿÿÿ»Ÿ®äl2¼/`¤ÂJç®+b¢)È¨ŠFò8äÊÉÜÇârÑÜ®9z="®ê [à;“ÊóG±«aªª*Ê6@¨*?ªúšµ™�#z*,n«cªª.‚ˆaˆ.
+‘°I¸«WŸ‚¦–¨‰ˆˆövkd§…``VlnA�™Ø¡Œ
§†6gDcªŠÎ‚ˆaˆ"
+k+‘°Ix«WŸ‚&¦–¨‰ˆˆ¶vëd§¥›�d&v(b©á�™Ø`Ù�6æ¶c¹~„Ÿ›še•Ô˜ªe¦¨n†g aƒf4Ó™ˆª“øúÙÎjj:	â®¨±ŽßâŠ‚)ë�äA¿ÀŸ±jÿ+WjeJºQ¡oŠ<.Œa¦Ù–®(Zr”Ø–Z]‰caÊja
©†‘/Íi�©g1‘WU™35yV›30‹nbžFÀZÉm‚Ù8«â!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
ŒšDÓ£×`�7îËžõmQ�.ÑGÕšêµÕY°ûìö�Ó›‹zÛUIABùÕ�m£PT9²µû� ¿T`÷÷M²TQÊõ÷.µMH†]‚Üw».{ÔYµFt»EThGª×T	fÀWï°kÚ?Nn-ëmT$U7çV=¶ŒeAPæÊÔcî<t‚ýQ�ÿßÜA6ÈQG+×ÜºÕ5 hb»Þ”ÝoÞo¬Ô~¾æe+jÚ�w8VßK»¹Õ¹»»«4oÀXFaU>·ÌrâÄR®JÇL»Çá�;°@ÅìŽ­:Æ$2bøÀ•“Œñ‚®uFÊb¦Ãz�°ó;ñ¯#¡Ú J“>š6ïÏ#t·(Ô~>ñxüâ¹×ÂÌæ1³Œ<n“ÑÄÀ¬?kÍAl-gyØ2¶c1£†ÿb&Þ^!�Ç|´Œ¼³4;±„áŽ <AãüRðß²70jÃ½u‹áÁÖ6]0¶‡�1b°¿YÄ,õÛ®¸ò
‰�4ÑÌç¬LÛÁ:4=Ç¼™Žóh.‡×¹´N‚‡jÆÓ 3@±Œs[/Á<‹'ulüÑLÃË�ñƒˆ‘Iìñ’|sý7²ñTVD‘�âÆÇS±*ïe–"Jý@Z@¯™¬íˆ¡6eþ’bj&¢Už¹µ•jå¯•}VôžU½ÿ–°ÊZÒv•\¼Ö"šR‹”ËZŠ�÷Y›ãßOO];ü|4ì7U·ÞÆt4ý´¼´ÀDò‰C£¶)ñŽkyN<
…w³	6(h—tL£�ˆmó
µ[òÆyŠg=z…]z…Tâ«‚jPªE�At… JË¢™i:èn¥g‰D–VU„Y·ÁhU^YóŒ•FpPX»ã”&e8 V~f¥V<&Tß˜bÀUaŽ`àPTãâ½P¾7¨\68[ŽØHCÆ¾£°Q&auOáTi±8˜Á#mêÞöÇfÁ¢þgN‘ÓÎ“Å iéáeÆàñˆ1W€QG{Ú•ÌÄâaª¥GSñD¢ê¿pÇàÓ]ºˆT`Õ% êhn€E0Ð0u ˜v�®2ð±´°I‹šªž mO†€EYViš§�…¢©'äXØ}Vþ—!*²½á2Ñÿ$UT®„oª«ŒÉn8èÕÕU÷Î}0‚¢£ÁµŸ^ê¯%àëM4gÕž/®H“É)}ëQ{‰7Ï�Ÿ³¸å¾)ôƒŽ= ÆRÈº|
µd§Á”3L, 
ÈÝÓôè�ñxF~tÈNa3Üëñ@ECBo	L4ÐDÍÄëÐ\ø&êd¾,
³ß¸z,j¢ì´ 0O˜\ô×0ú©Â¾ª8>¨íØ®¯Œ¦ÿ†¸�—É§Ï¨HNó³Í<\|LÓÒS>Âj»éÍ¸Œ&­ß6`�îÒu¸îód¥=&w¼æÎrØ8ÏñßÏ]üáÌþ‹Žü6ó§GSˆ|»ÀN6"Ñ
­òÛ|W3Ð#èb<0± ÇÃ¢ó?‚F�ƒÉOj¡0ôÃÌd<sÃ É3L|P$3ª�;¬8Œàôt¦7CÐÀªQ*–R©Q*�ÁU³ê«º¦m-éØˆ|+õ‰Ã¨
ýbp+Â¿Xœ:€¯Ö'#›ÝD~«ª¿ã�5¨þM¸ï'iðÁŽ³ûBŒ$Í‡�lO�óï¾µlžðÐ¶¡üm:3p…t^º»Ã8÷Œ¬‘²è]}¸:ãèøÃ0Èô0‘pñ�±µT–À!|´Ü¼
èúâ³	ÛÖÓ“±»žðóð«;©Æè“[Pë°gË}<®ƒë¬œã`×# Š—Å�
¹ô,º¸êóÛÏ¡'/5È�Áw0ð:.þ¿¯Âl+`k
F_=´ŸBÉoÝÞcÃÃøÁ_¹Èü¸ì“ß³Ó²ÂÜëp÷/aŽ1æÌýËdM°\U[‚ŽË? 0ëå±Ã7Ã�(ðê0kL<è÷ß Èë�±Œ£ÎŒÃë^»ßBÛLó<j'Àð`¨ÇËsÆ¾Àè ¯Hö�ÃG_ÓÎœø=|ü¯½Ý€6½Ã,#à¢º¯cÏ¾¤ó•kn{jkj;j{‚N3šÑ“NÂX.� Éó'n´è"ï<5áR‡ó­ƒƒ"ãÐàõÊ4ú8ã#;é2*#ª;j»©ûi{V¨jZ´ÃÓ>ìÀÓ,þÀ®Ô\(�0Ò>NÜÎ2À{Q`4ìŸÃÓ>Ã_·ÏÌ�¨lüÇqÕ:$úöòµŒã¥ôï} ý˜²P:7Ám7°ãèlÁÝ=KÏ> ÎòÆÞqK3ÃÆ­Ë0µ²ÀÂÆ<uÂ“
¯ÍÃÏÖÌãø°[]l?Ã�09
1‚Ë£áüú¸)IÓ~¶2¿èÉ¨©©í³m™Í1óaÍN*žÕ�Û~ªG3°«­0õ8<==:´u|=­�<Ü(G#00ì]Éü<ÆÛ¼ûºÂXUp¬¾/‚0«c¢¾è2¶»<ô°ûXËíÁC|Ï>°€Ëzû8ÎÔŒÂtÜõ4�<û«pŒ>¾NÌŒÆâ<$ õzÜûè0û3ó¼ÂÆ@\ì^ÃXðÅÜ-wØÃÃÃ ‚,?pî=sCó<>øÎÃX½$,ÀŽ0=.O‚Þ-×,ô�‚ÔÛàôï3à�n»]:ÓÃÌ�+Ê
Rø,°ŽÀÙ?ML0Îè­ƒ%‹¼+’ÍI®z˜Ç•w1ÔUÏAT>,ÉzAèŒv,ªÑG7…ËE<xÞdÓ�µIêñ _â
�ÙÕ�ÅÁ•ú­Ïê•›Êk–W;ªî�©üõ™¾-]á’¥¯Æä—daß31–¦ù*39š¢«›2iX™hÀ{–ÙUý»eeËciØ¥¦�Ž—%­tV™†[lÔ|5Ðk[¥êhõb¡q°k‚ß‰kw ‚ ƒ“¢£ïãŒ*­ñJØô½2ª4ÑTóÇ¬*0³oêè§Ï\Íf>ŒBÍVÃ¯gâ·Ç3‡1µG0†voOcw†{­ïv,"v„�?\›Üòka–Ã†4«¾=E+ª½'ë¸ò¯VÏ:�ÙöòYp­ÓvÕ³†	°ˆL³EÚv‡4!°s,§2Ž¼ôƒ1Y¯Èß£o
Êg½Û}Î€‚ª-ÌæÓº–Ï‰HÎŽ¯ò‡�ºÎ'JêÃ¢�Î±_XùQÓ…O4Ò­µ·ÜcØ­¸ý,M»­2P%ŒBp�Jõ½/ic ²7ÛMÊl[ê=ÉKÍºØqõOIREÂ¢
[Ax þ·�x|ñÌSFmâàêƒˆzÄ­‘~·ð£ÑÁ­÷£T¿9#è½(×ßŠÍ·4­s‡möƒß~=-ts §K£1ØË}Â²÷ô"°È"DÚsŒ`kÃd¥Em�IGŽªèò«,%·Úü0.=+JÊ+wWg'z¯ÃYvpÈvÃ­X2dóøc½·¯†šÝê<–6ÅsMÆsƒãõú°™Që3†}£óe§n6Ã†ÊUãàÝ5£ðêè—3«÷×8mkÝ*³Ñác÷CËª†­À 
¹Ðpq,°®%æÛm¶Gw•%´i´¥NbY©ßeõµ¥U‘´©QQµNÕU•Q@¾_QVUäV§ûF  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,º<?xml version="1.0"?><Features nextValidID="23"><Feature recentID="22" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3107-3123" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3083-3099" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12055" maxFusedRunOn="12055" detectionMode="exactProteinMatch"><Segment range="12061-12078" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3176-3197" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11728-11752" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5450-5474" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3131-3137" color="#ffffff" type="standard"/><Segment range="3138-3155" color="#ffffff" type="standard"/><Segment name="-35" range="3156-3161" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7554-7694" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5198-5372" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="13903-14092" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8038-8232" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="11774-12026" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2813-3065" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6780-7368" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="9799-10428" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3388-4065" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="5899-6693" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q></Feature><Feature recentID="3" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4133-5158" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="8298-9365" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12055" maxFusedRunOn="12055"><Segment range="12085-13902" color="#993366" type="standard" translated="1"/><Segment range="13903-14092" color="noColor" type="gap"/><Segment range="14093-14101" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  B<Notes>
<UUID>7c02b138-7d78-463c-93da-a3af8b32e764</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:33:38">2024.7.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  4ý7zXZ  æÖ´F !   t/å£áV[ó] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷Ànþ˜{ }åµßöÓ•>£ÔEöK½ëoaÿšUÉ›Ië&@rqõ×GVý_Ÿ4îŒè¬4¨X¼LÞ‡D½ñÖº®Á^3Gë ÄáÆÛ½ÎV±v˜¶&œ^gœËÍI¦é1ü ¦zLér>övªòÁ…þ6!E±�›°oˆôŽì´˜h¬aaœœ6$ªîÏ5á)FÔN™gW=Gæ@rÀdÒ3R[rA5¥	(m÷§¦Œ¤]©,L¡ÅpzÆ³Ä…` •æö;¡ÚùðÃ?,Ã”Fp6¶œA®ª{ç4®e–¤QQàA«b+	,È®cñfD3w©z û—ÑÝ_k³³VŸU2Kúš‹B�Ü¯§à²–¾ ¬#G�0Œª­1«°ëÿí"û1·¦Š��\‚.†Í§®{Ø?ÒÇ¸’ë®¸X;¸îóõÖâùT5™¼nq‚ô=E©mÊáòJ�C9¯ÑaY‡¡S5;Ùð£>p~”×².q¹ÌfKkî¬
ä’µïÓÝ”~;3è?âòZ}ßAü›ðt}Éq½�â÷Jö°²kKL©L\YXöæWÁ�ÏŸ¶®·R¡9^¹c¡è¸lž^&•c†Ü§ªg?à3Ks‹ÞTV ;ß²’}Eï» *œ8cX¿ã	£-- ™€â@m µLOxÆX™ù	—û5ø‚ä�°þ6Ö¾ºé÷}n_´l•kZ€g-ïã¸/’Ø¡»¾Åag¡ÅPUà�kÂù;*]VÌ…²ÇÛ¼£ì�I¤E“ü�„7Jû¨RÏ¶‚»0`I|!ú�ä~¦±ópDÚÁéïo¦�ü#@>ÊKü~-Ž=e~üi¨»�ÞbAÊZ9­Ù©´½÷Ð·8Um?�ÜwU¬ÈÞsÜA±AÇz AÓ!ä�6×¸ã�ê`"BãUï[cA·—Å÷‰ç0n“åñcI�î]>Ð¶Ïº5ÂíIêçxîh*ÜQä„ÜÇ(8›„qtÁ*õÄ÷v³º�%hqûN… s­U&h´œì™¯–W‡ŠœXÿ!šcB5Kôµ®Ô“R!eš��dÝÅ˜æãGn˜ãJ	Œ›ðf½äH�­Yq0ª�Í 5t|Í”âD\+rFGÑ½GnÔËÒ š��ö�¿¾ÿ¬Hà‰´ÍFðÂ¥°yãØq)ÖF«\î‚ÆäÕ‹¼…@½ëË³Úû&Ì^2QÇ¼—bÜm¿Ç&
ºJÞP/ÐÐÜ±‰BÛÚVãä‹‡É"µØ¡.¨·Ãý|ÅÈƒh�Æ^›òg<dÚ„@ô‰õƒQªòÖþëís7ªœk�&ºEGËŸ¨Ú'w+xœzÐ­Ž}l#^WóœàÊs¹*ˆ+ÖX!˜ÃKÑ,¼“}àyó`³“îhè�s’ÆØý!S©TÃ3äÏ]´å{{/;ÖEÓ^f�®¶¯%æ³w>vÙW@ËxÆ^¸Bíäf„©Ú¤4î]?£k°`9b‘D+ -ûWÖ‹#–¸ÌÇ^1QO�-ý5­Ëà#0{äO	`’ÅÉì‡Ÿ)-¹‡óI9õ NÄì‚ÅyîÆÕôXF~ùÄÉ'¡R®Ù‡'bQè›„ q4|Ex´‡ð²’‹ë+þX•è®ÛÄæÁ,è]&×à¨æh¯Ø¤Ö[äW@Réa‰J2VgÄ‘£‹àhä¼#¡‘ƒ—<¶b4¡šjà›ô£s¦/š{˜Œ>+”e¿<(Ð‚[*ãr­î”)íÞ ÆºBvÔ»Šðß‘Z�Ç‚â²äâÅ÷-;G:lîoWÏc˜ªÛ´¼›¿jîã%þoÊÎ!ÚØXpƒ+R)‰3t€Ç�H?»ä¨	äGî¦l UP˜õ-Ë‰œ:¼ÕV«‰Ö|¿ï=X Î&µ.Õ‰Y�ÍÔˆ¯g‹�(�røÆBÇÆßr·QXãdO£Êª„éÌ¼¸Nîöy²B×_NS½Ø¨7CôHÖq™¨SnX5
/‘â&Œ×KzÚ]< ¦{…³Ï"’yY„pû`‚Î·õ�]&‰É|Æÿ'•F€àÑèRŽ{mq‰ý2—D·ŸSuc†·V£¬h“˜n£Šw4R³½^R7Ä3×ˆÿµJ„œ ‰qëA¢µ%o0LO¡ñÕÙ×÷fÝôˆÎ¢qèœ¹ÿ°{Wª:|CNMHˆPœYùŽš;ý‡w³,)&#Ö×¥ÊA?Á–\£šä¼œøz?~µŽîi˜l² C»¥ÇÚ%æ¿jVÌüº	Ó'ã†rÿúO°Í+å.,KÑ“BY÷kÂgžå”àñ×åšôÎi“6+#“PFÃ•T–|XÁx1ÐPAOòQDû^ò„KZl,êÙdI2Â_<Qj¤�tÆà”ðHÎ@ÊkÄ"˜ÍË³!t†¡Øë{«ÜŽj“f©b4š^€‰A¦ò{–…?£}|¿Œ–“R¥[…ð`ç¼wï—X�°@Ñqu9ÁÎÏæóÞÓ‘Ãð.7²-èrHpqáT­ oô7<Þ-ÜO¸"d¸_F@Oàv%#q|·Ì�%\‚Øî€ }Ø[.ÄI	­§GOÀ¾Õÿ)Û*B£ÄåpÝë/kówI‰È¬?_½ìøŽëâ�1=}Šäü×¡ˆ¤ï‹'“¼a2C¡Aé)$fÆ›–—ù6¸6m›Ðöøç%9‹}&ÉpB'$<sû‹�¥¦ÍTÊ÷Yµ<ià7Ã�žŒáüÃ†	PF~HZè‡©u1{ùJ\“�ÿeþÞB9®¶È+üNóÜD­æ›ë>è§Ûh)V$¸t=ˆi’�¬¶²I“O¥U˜f¿ÍUQõB”Ýß‚—[£ é
K¾²²ŽÛL¹°ÊØ °)&ðjFÞ½
�»ŠÅùÇ_�9Ô¶ÇCXßìífÎ¢}Úp.vúsm+ÐY®Ja¤Ó ñùûÿ‚˜:-þ\�ß{78Ê'_€‰fšà˜˜çNü0@«ˆ/%m#¸È¹t†Ö""®³‹»ˆÅL©B¸ã©ñ7Øx1|-ÿ\Ø}dgp÷ÒÎû0áþb1d-Èô†ÁÎd™™<bú0`¹÷øÓédÅvcßÖiâa
3É-&ÂNµæêÐ`SÐçÜ�ºòwù5Z¡!äJŒo‡-Zß­­áMé{ì•¦ÇÜÚö<ºÚÕö{ƒÚJáûJÀï¥üZó	4æÄ*�Ìah'~Â7¿ýI.B«¦ÐŠüØ�9cº˜�ÑN«_Âêý—':ÌdÓ)�:–Á…ÒŽè®GïïªUÒU�ÆL„	øÝÉ€©‘)J&a�Óø±¶`¬œ�÷£Zœ¶¶¯Û³þ�Ä®~6/a¦ØÓê|«VQ#åh\˜Ü…7?‰š$
ß×¶:© £ÒgU²Vè6ÏPhè †Ž“ËÖê÷¨–¤«3s§H´„ï]ø0ªlƒ¾_«ORœ±¹x÷š0rWêÓ2—H¨xòŸ5¦­>¼N/Q	¬¿ˆÈÊ˜Vm<ë"1Ý�’iî·®c'„eç?¢‰©'‡WÞú¥«±Îî©·&½þ*ô-WùWŒ+È"&µy7­	¯/°qpt¸<\`¿ù«|Ê]€n û«'3R>G”!VÊ¥ŽÈ—¢ŠÖA±yí�gÔÎ‘>Þ“Å<29Œ\ïÂC¿¨µ¹&wŒKjpx—V0Œ‰µ°=”÷´Ñ ïÀC¨-X,eÎñy½±lÈ'„P{ã²A’L¬`9ºêùÿxábæñVà\�®)óÀ.…_úaôÂ©¨”Êz~¸>_¦7	MçKŠ¹Àa£Îç�õ�f¦
	@ G5MOWØ'wCS£`9¼yQK<«wOÆžÞÒ—Ó[Õ`aƒ™ZèÙ,{íYS‡“©û1¢ªF¹É9á^d¸'p	ñ˜é„™KÜØêïWŒýç¶à€t�ÄÀìtüËìªÇ:xPýM1žhWü^`ïX;-X¾²ÓÇ<µ�8vIdÉÄZá~hÊíî@ûS Rp¹ÿTß¸{.Úå°à÷ìEµŒ\Ãf´0üÿµôª= ìãýA#îÔ-ÌQÒ�)ü$45¦B©þ,)­ÿZMj•O0 )÷w¾„L¾(aÕK·6ˆˆf™@¿Ë°±%´2 YvÞãH¿ÙQþˆ¦¯îŒ>��»‰ø!³d¨„$g½BmË§�œßï‹ýoH'Èf²Á¸¸Š1~'zÈ›¡Ináv‚ê¸<õÈû}ÿ	m(”%!Ühûž[¤ÓÚN_§À®¼r¦ÍÍÔPàøžµ“ÞNUÿ®¶çÒ�äsU˜ ôªqRŸî“o—Ÿc¾Ú¦+ùÇ¡WpÂš‡s½éßÂfÈ\ßiÏó=¬ñç{[¤ÍÀ•×Š©ˆU·zÎ›eB2&M—ZPX¥p0ù]ê„€àp·5*&Ë¢¥q¦B.s®·=®z©ýñ™6g¥
¯F�5ôäÆ�[Æ;Œ�LÒï_~ü¤ÔÆûø¥aX4ÝT‘Öþ·ìÇ�øjí}6^LH4KÜ.æöÄo R?ø™:"Üþûwì¡ï�qªU®ºÍx�3lÊ±XËê”¾ˆU˜¨NFÀPðo³õiŠ¾‡ç
U³œƒQ´$».Û¤´»ýWçS:ÈË·1g~g©™¡Ñ´/*œî˜™KûãÐ›ŸM.DÃD\iÄØ Áò²çÌûûyráö-SA¥c]{�PÓ“ ú–÷êV‡éÆäM8‹¼|è‰¼Úýn(´l-I ¿>¸‘pÜÂ½é=©@(¦ Æ‰�ë[	xe}Ù�·¼-e¾¤–‰ù<i›§ª8¯ÍùA~á\«Ú4v¿þo�	„h á›«`çÎû@4[u�~Ô]œöÖß°äÚQ!ŠË+Ò¨>)gÒÿ®ìÞí#7v9›Mnù ½c^‹àÂ€9[„>‘äyã>`ê·^±¬×]&v€Œ¥=îjMãˆNXëoU	(›¿�É²D5˜õño¶†é[ú2xòoñIy°lÿÒ‚žÍê‹{þÊÞW9é3¡XÏ‹–X£3ç3yü%õz(Ä‹üv…ÑYØ=bO„ùû&œë‹‚Ì]©^Åö�b†Ã¬3.”A.ŠÚhøsàó²”óaÆ®;ûQvE6×‚èØÌª¸°Æq^[~Hœ\#º	Ž\ª°ðbÍÿÏ×øö£ÀÜ‰õð|×/1ÇžÐ@Óµ�Nž^2z6­5è*÷í Q :ï“–¥Øàj\¡�Þº´ëw{S©ÿ"±•µþ{�@
 �Ý?`#Ôö~¦‹zòfò@<…Þ	J}ýî‰sÓLvÇ£m.aò¸ ñÎÁÅFÄNÌå¨˜¬ƒQ€2—nÓ¡ñkÓGÿåS†¼Ýx§sYÍ	Y��|�ø�Õw&��Ç <‘éµîe;ï)kw(ÂªWjÓà=¢l$IˆuÆÈ×¨tõ‹íµ] ÛJ‘ÌÂ&*ig;fýµ—2�é5ÄmŒ )Ò¸((ß:¯{×Ðž¤´AÈæç–cÌk½êÊ=…YsM¤S1_û¡7ËW×¨_Ò7(’zZJÏM"îéÅEîqzJ„E)M§ì_ß·~6àœ£rS8¿EQ…1’Àíª~    rXº¹ÍÆeY �"Ü¬ ÔX%V±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  :o<?xml version="1.0"?><Features nextValidID="25"><Feature recentID="0" name="lac UV5 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3464-3470" color="#ffffff" type="standard"/><Segment range="3471-3488" color="#ffffff" type="standard"/><Segment name="-35" range="3489-3494" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli lac&lt;/i&gt; promoter with an &quot;up&quot; mutation&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="ccdB" directionality="2" translationMW="11734.81" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2807-3112" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;ccdB&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Plasmids containing the &lt;i&gt;ccdB&lt;/i&gt; gene cannot be propagated in standard &lt;i&gt;E. coli&lt;/i&gt; strains.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;CcdB, a bacterial toxin that poisons DNA gyrase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MQFRVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI*"/></Q></Feature><Feature recentID="2" name="GUS" directionality="2" translationMW="69250.83" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" maxRunOn="12779" maxFusedRunOn="12779"><Segment range="12809-14626" color="#993366" type="standard" translated="1"/><Segment range="14627-14816" color="noColor" type="gap"/><Segment range="14817-14825" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;This version of the &lt;i&gt;gusA&lt;/i&gt; gene has a 5' extension with a catalase intron to ensure expression in plants but not bacteria.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-glucuronidase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DLR,,NRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"/></Q></Feature><Feature recentID="3" name="Ubi promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="778-2769" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;maize polyubiquitin gene promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="pVS1 RepA" directionality="2" translationMW="39695.35" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1"><Segment range="9022-10089" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;replication protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"/></Q></Feature><Feature recentID="5" name="HygR" directionality="1" translationMW="38032.99" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4857-5882" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(4)-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to hygromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKK*"/></Q></Feature><Feature recentID="6" name="KanR" directionality="1" translationMW="30974.66" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6623-7417" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aphA-3&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="aminoglycoside phosphotransferase"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*"/></Q></Feature><Feature recentID="7" name="CaMV 35S promoter (enhanced)" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4112-4789" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus 35S promoter with a duplicated enhancer region&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="pVS1 StaA" directionality="2" translationMW="22059.04" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="10523-11152" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;stability protein from the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSARDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"/></Q></Feature><Feature recentID="9" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7504-8092" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CaMV 35S promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="23-368" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter from cauliflower mosaic virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="NOS terminator" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3537-3789" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="NOS terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12498-12750" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;nopaline synthase terminator and poly(A) signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="pVS1 oriV" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8762-8956" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;origin of replication for the&amp;nbsp;&lt;i&gt;Pseudomonas&lt;/i&gt; plasmid pVS1 (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/10659714&quot;&gt;Heeb et al., 2000&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="cat1 intron" type="intron" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="14627-14816" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;castor bean catalase intron, modified&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CaMV poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="5922-6096" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;cauliflower mosaic virus polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="bom" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8278-8418" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;basis of mobility region from pBR322&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3855-3861" color="#ffffff" type="standard"/><Segment range="3862-3879" color="#ffffff" type="standard"/><Segment name="-35" range="3880-3885" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="LB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6174-6198" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;left border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="RB T-DNA repeat" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="12452-12476" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;right border repeat from nopaline C58 T-DNA&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3900-3921" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="12779" maxFusedRunOn="12779" detectionMode="exactProteinMatch"><Segment range="12785-12802" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="22" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="745-761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3807-3823" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3831-3847" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ™<Notes>
<UUID>3825558d-0fc3-4d19-82b8-74c45b2b3d77</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pUN1301-CCDB</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="10:34:36">2019.1.11</Created>
<LastModified UTC="8:59:21">2025.7.16</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO ÿþ              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="2796..3513" colors="orange"/><ColorRange range="3514..3519" colors="blue"/></TopStrand><BottomStrand><ColorRange range="2796..3513" colors="orange"/><ColorRange range="3514..3519" colors="blue"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
